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2 protocols using phloridzin

1

Characterization of Apple Peel Polysaccharides

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Dried apple peel, as a by-product of apple chips, was obtained from Meiqingyuan Food Co. (Sichuan, China). A. oryzae (CICC 2339) in lyophilized powder form was acquired from China Center of Industrial Culture Collection (CICC) and it was kept at 4 °C until use. The standards of mannose (Man), rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), xylose (Xyl), arabinose (Ara) and fucose (Fuc) and lactose (Lac) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). High-performance liquid chromatograph (HPLC)-grade chlorogenic acid, hyperin, isoquercitrin, ellagic acid, phloridzin, quercitrin, quercetin and Folin-Ciocalteu’s reagent was obtained from YuanyeBio-Technology Co., Ltd (Shanghai, China). 1-Phenyl-3-methyl-5-pyrazolone (PMP) was obtained from Adamas Reagent Co., Ltd. (Shanghai, China). Acetonitrile and methanol of chromatography grade and other chemicals of analytical grade were purchased from Kelong Chemical Reagent Co., Ltd (Chengdu, China).
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2

Fuji Apple Juice Production Protocol

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Fresh “Fuji” apples were purchased from a local market (Zhejiang, China). The apples were washed, peeled, and the cores were also removed, after which the apple flesh was cut into slices. Immediately, the slices were immersed into 0.6% ascorbic acid solution to avoid the enzymatic browning. Afterward, the slices were squeezed by a juice extractor (JYL‐C022E, Joyoung). The juice was collected and filtered with a 100 mesh filter. After sterilization at 98°C for 30 s and filled in brown glass bottles, the juice was cooled to room temperature (25°C) for further UF immediately.
Folin–Ciocalteu reagent, sulfuric acid, phenol, ammonium acetate, bovine serum albumin, and ethyl acetate, all of analytical grade, were purchased from Aladdin. Chlorogenic acid (>97%), caffeic acid (>97%), catechin (>97%), epicatechin (>97%), and phloridzin (>97%) were purchased from Shanghai Yuanye Bio‐Technology Co. Ltd. Coomassie brilliant blue was purchased from Shanghai Baoman Co. Ltd.
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