Human tdp 43 hs00606522 m1

Manufactured by Thermo Fisher Scientific

Human TDP-43 (Hs00606522_m1) is a gene expression assay designed for the detection and quantification of the human TARDBP gene transcript. This assay provides a tool for researchers to study the expression of the TDP-43 protein, which is involved in various cellular processes and has been associated with neurodegenerative diseases.

Automatically generated - may contain errors

Lab products found in correlation

Purelink rna mini kit, by Thermo Fisher Scientific (2 mentions) Mouse actb mm02619580 g1, by Thermo Fisher Scientific (2 mentions) Iscript cdna synthesis kit, by Bio-Rad (2 mentions) Itaq universal sybr green supermix, by Bio-Rad (2 mentions)

Close spelling variants of this product

TDP-43

2 protocols using human tdp 43 hs00606522 m1

Quantifying Ataxin-2 and TDP-43 Expression

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

At 48 hr after transfection, RNA was extracted from cells using the PureLink RNA Mini Kit (Invitrogen) and converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturers’ instructions. qPCR was conducted using 20–40 ng of cDNA per reaction using iTaq Universal SYBR Green Supermix (Bio-Rad). Measurements for each biological replicate were conducted in technical triplicates using validated primers for ataxin-2^{97 (link)}. Values were compared to GAPDH, and the average fold-change was calculated using the 2^ΔΔCT method. All primer sequences are provided in Supplementary Table 1.
Tissues from injected animals were dissected and RNA was extracted and analyzed by qPCR as described above for ataxin-2. hTDP-43 mRNA was measured using the PrimeTime Gene Expression Master Mix (IDT) with the following TaqMan probes: human TDP-43 (Hs00606522_m1; ThermoFisher Scientific) and mouse Actb (Mm02619580_g1; ThermoFisher Scientific).

Zeballos C M.A., Moore H.J., Smith T.J., Powell J.E., Ahsan N.S., Zhang S, & Gaj T. (2023). Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins. bioRxiv.

+ Open protocol

+ Expand

Quantification of Ataxin-2 and TDP-43 mRNA

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

At 48 hr after transfection, RNA was extracted from cells using the PureLink RNA Mini Kit (Invitrogen) and converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturers’ instructions. qPCR was conducted using 20–40 ng of cDNA per reaction using iTaq Universal SYBR Green Supermix (Bio-Rad). Measurements for each biological replicate were conducted in technical triplicates using validated primers for ataxin-2^{101 (link)}. Values were compared to GAPDH, and the average fold-change was calculated using the 2^ΔΔCT method. All primer sequences are provided in Supplementary Table 1.
Tissues from injected animals were dissected and RNA was extracted and analyzed by qPCR as described above for ataxin-2. hTDP-43 mRNA was measured using the PrimeTime Gene Expression Master Mix (IDT) with the following TaqMan probes: human TDP-43 (Hs00606522_m1; ThermoFisher Scientific) and mouse Actb (Mm02619580_g1; ThermoFisher Scientific).

Zeballos C. M.A., Moore H.J., Smith T.J., Powell J.E., Ahsan N.S., Zhang S, & Gaj T. (2023). Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins. Nature Communications, 14, 6492.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!