Anti-rat CKLF1 [Genebank:NM_139111.1] neutralizing antibody obtained from Peking University Center for Human Disease Genomics. Antibodies against ICAM-1 and VCAM-1 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-myeloperoxidase (MPO) antibody was purchased from Abcam (Cambridge, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Pierce Biotechnology (Thermo Fisher Scientific, Rockford, IL, USA). TNF-α and IL-1β ELISA kits were purchased from NeoBioscience Technology Company (Beijing, China), and MIP-2 and IL-8 ELISA kits were purchased from Westang Biotechnology (Shanghai, China). MPO detection kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other chemicals and reagents were purchased from Sigma-Aldrich (St Louis, MO, USA) unless otherwise specified.
Anti myeloperoxidase mpo antibody
Manufactured by Abcam
Sourced in United States, United Kingdom
Anti-myeloperoxidase (MPO) antibody is a laboratory reagent used to detect and quantify the presence of the myeloperoxidase enzyme in biological samples. Myeloperoxidase is an important enzyme involved in the immune response and is found in certain types of white blood cells.
Automatically generated - may contain errors
Lab products found in correlation
Sytox green, by Thermo Fisher Scientific (2 mentions)
Mpo detection kit, by Nanjing Jiancheng (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Cytochalasin d, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions)
Anti histone h3 antibody, by Santa Cruz Biotechnology (1 mentions)
Dnase 1, by Merck Group (1 mentions)
Percoll, by GE Healthcare (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Calcein am, by Merck Group (1 mentions)
Anti β actin antibody, by Abcam (1 mentions)
Cell proliferation reagent wst 1, by Roche (1 mentions)
Dichlorofluorescein diacetate dcfda, by Merck Group (1 mentions)
Streptomycin in saline citrate buffer, by Thermo Fisher Scientific (1 mentions)
Hydroxyethylpiperazine ethylsulfonic acid hepes, by Merck Group (1 mentions)
Tumor necrosis factor α tnf α and interleukin 1β il 1β elisa kits, by R&D Systems (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc, by Thermo Fisher Scientific (1 mentions)
Enzyme free cell dissociation buffer, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti myeloperoxidase mpo antibody
1
Neutralizing Antibody Procurement and Cytokine Analysis
2
Immune Cell Isolation and Functional Assays
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Percoll was purchased from GE Healthcare (Little Chalfont, UK). Phorbol 12-myristate 13-acetate (PMA), DNase I, Sodium hydroxide (NaOH), cytochalasin D, ASA, DXM, Calcein AM, and dichlorofluorescein diacetate (DCF-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-histone H3 antibody, anti-phosphorylated nuclear factor κB (anti-p-NF-κB, p65) antibody, horseradish peroxidase (HRP) secondary antibody, and secondary antibodies coupled to AF488 or AF555 were purchased from Santa Cruz Biotechnology (CA, USA). Anti-myeloperoxidase (MPO) antibody and anti-β actin antibody were purchased from Abcam(Cambridge, UK). SYTOX Green, Quant-iTPicoGreen double-stranded deoxyribonucleic acid assay kits were purchased from ThermoFisher Scientific (Basingstoke, UK). Cell proliferation reagent WST-1 was purchased from Roche (Basal, Switzerland).
3
Neutrophil Activation and Dysfunction
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Reagents LPS (Escherichia coli serotype 055:B5), formyl methionyl leucyl phenylalanine (fMLP), interleukin-8 (IL-8), phorbol ester (PMA), cell chromatography C (Cytochrome C), superoxide dismutase (SOD), Elastase, Hydroxyethylpiperazine Ethylsulfonic Acid (HEPES) and Emodin were obtained from Sigma-Aldrich (St Louis, MO, USA). Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) ELISA kits were obtained from R&D Systems (Minneapolis, MN). Anti-Myeloperoxidase(MPO) antibody(abcam, ab65871), MNase, RP-1 antibody (BD 550002), SYTOX Green, and Annexin V-FITC were obtained from eBioscience (San Diego, CA). pHrodo Red E. coli (Cat.No.4615), pHrodo Green S.aureus (Cat. No. 4620) were obtained from Sartorius (Göttingen, Germany). RPMI 1640, fetal bovine serum (FBS), trypsin, and enzyme-free cell dissociation buffer were purchased from Gibco (Grand Island, NY, USA). Penicillin and streptomycin in saline citrate buffer were from Invitrogen (Carlsbad, CA, USA). Other chemical reagents are of analytical grade.
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