All cells were confirmed to be free of mycoplasma infection using PlasmaTest (InvivoGen, San Diego, CA). Wild-type EBV was prepared from supernatants of B95–8 cells cultured in RPMI medium 1640 supplemented with 10% FBS for two weeks. Briefly, the cells were pelleted and the virus suspension was filtered through 0.45 μM Millipore filters. The concentrated virus stocks were aliquoted and stored at -80oC.
We infected ~2 x 106 Ramos Cells (ATCC CRL-1596) in the presence of growth medium containing 2μg/ml of phytohemagglutinin (PHA) for 4 hours. The infected cells were washed, cultured in growth media, and observed daily for multinuclear giant cell formation and morphological changes characteristic of EBV-infected B cells. After 10 passages, the infection was confirmed by measuring the expression of viral EBNA2 protein levels (Supplementary Figure 6 ).
We infected ~2 x 106 Ramos Cells (ATCC CRL-1596) in the presence of growth medium containing 2μg/ml of phytohemagglutinin (PHA) for 4 hours. The infected cells were washed, cultured in growth media, and observed daily for multinuclear giant cell formation and morphological changes characteristic of EBV-infected B cells. After 10 passages, the infection was confirmed by measuring the expression of viral EBNA2 protein levels (