Immunocap feia system

Sera from 9 patients admitted to the Araba University Hospital (Allergy Department, Vitoria, Spain) with diagnosed A. simplex allergy (but not reporting previous anisakidosis or Anisakis infestation, according to their clinical records), and presenting high levels of specific anti-Anisakis IgE (higher than 30 kUA/L) (ImmunoCAP FEIA System, ThermoFisher Scientific, Waltham, MA, USA) were collected. These sera were mixed (by equal volume of each serum), and this pool was used for specificity in vitro testing.
Negative control sera were obtained from healthy individuals without any allergy symptoms (2 patients) and from patients allergic to other food proteins but not to fish or A. simplex (10 patients). These patients were tested by skin prick tests (SPT) with an A. simplex extract with a negative result before using their sera for in vitro tests. Sera were tested later (in vitro tests) in two different pools (one for non-allergic and one for those allergic to other proteins).

Allergen-specific IgE was measured with the ImmunoCAP System FEIA (ThermoFisher Scientific AB, Uppsala, Sweden) following the manufacturer’s recommendations. Venous blood samples were analyzed for IgE to nuts (peanut, hazelnut, almond, chestnut, pistachio, pine nut, and walnut), as well as to Pru p 3, Bet v 1, Phl p 12, Ara h 9, and Cor a 8.