Dynabeads m450 cd19

Manufactured by Thermo Fisher Scientific

Sourced in United States

Dynabeads M450 CD19 are superparamagnetic polymer beads coated with anti-CD19 antibodies. They are designed for isolation and enrichment of CD19-positive cells from various biological samples.

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Lab products found in correlation

Detachabead cd19, by Thermo Fisher Scientific (2 mentions)

2 protocols using dynabeads m450 cd19

Isolation and Sorting of B Cell Subsets

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Mononuclear cells were isolated from PB and SF using a density centrifugation with LSM (MP Biomedicals, LLC, Santa Ana, CA, USA). B cells were isolated by positive selection with Dynabeads M450 CD19 and DETACHaBEAD CD19 (Invitrogen, Carlsbad, CA, USA) as previously described [19 (link)]. As we previously showed, only negligible levels of artificial activation of B cells occurred immediately after positive selection [20 (link)]. To avoid unwanted further stimulation, cells were normally rested on ice prior to any stimulation. Isolated B cells exhibited greater than 99.5 % viability and more than 95 % purity, confirmed by flow cytometry. Cells were stained with mouse or rabbit monoclonal antibody (mAb) against human CD19, IgD, CD27, CD80, CD86, CD183 (CXCR3), CD254 (RANKL) and CD307d (FcRL4) (all from BioLegend, San Diego, CA, USA). Naïve (IgD⁺CD27^–), IgD⁺-memory (IgD⁺CD27⁺), double-negative (IgD^–CD27^–) and switched-memory (IgD^–CD27⁺) B cell subsets were purified by flow cytometry.

Ota Y., Niiro H., Ota S.I., Ueki N., Tsuzuki H., Nakayama T., Mishima K., Higashioka K., Jabbarzadeh-Tabrizi S., Mitoma H., Akahoshi M., Arinobu Y., Kukita A., Yamada H., Tsukamoto H, & Akashi K. (2016). Generation mechanism of RANKL+ effector memory B cells: relevance to the pathogenesis of rheumatoid arthritis. Arthritis Research & Therapy, 18, 67.

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Multiparameter Flow Cytometry Isolation

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B cells were isolated by positive selection with Dynabeads M450 CD19 and DETACHa-BEAD CD19 (Invitrogen, Carlsbad, CA, USA). As we previously showed, only negligible levels of artificial activation of B cells occurred immediately after positive selection [33] (link). Isolated B cells exhibited greater than 99.5% viability and more than 95% purity, confirmed by flow cytometry. MNCs and isolated B cells were stained with mAb, and Th1 cells (CD3 + CD4 + CXCR3 + CCR6 -) and B cell subsets such as naïve (IgD + CD27 -) and switched memory (IgD -CD27 + ) B cells were both purified using flow cytometry.

Nakayama T., Yoshimura M., Higashioka K., Miyawaki K., Ota Y., Ayano M., Kimoto Y., Mitoma H., Ono N., Arinobu Y., Kikukawa M., Yamada H., Akashi K., Horiuchi T, & Niiro H. (2021). Type 1 helper T cells generate CXCL9/10-producing T-bet⁺ effector B cells potentially involved in the pathogenesis of rheumatoid arthritis. Cellular immunology, 360.

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