After 7 days in vitro, cell-laden hydrogels were rinsed 3× in PBS for 5 min per rinse and then fixed in 4% paraformaldehyde (Sigma Aldrich) for 5 min at room temperature. After rinsing with PBS, the gels were permeabilized with 0.5% Triton X-100 (Sigma Aldrich), blocked with 5% donkey serum (Invitrogen) and incubated overnight at 4° C with anti-TUJ1 mouse IgG primary antibody (1:1,000, Biolegend). Hydrogels were rinsed in PBS and then incubated for 2 h at room temperature with donkey anti-mouse IgG secondary antibody conjugated to Alexa Fluor 488 (Life Technologies, 1:1,000). Cellular nuclei were counter stained with 0.4 μl/ml DAPI in PBS for 5 min. The labeled hydrogels were examined and imaged using a Zeiss LSM 880 inverted confocal laser scanning microscope.
Anti tuj1 mouse igg primary antibody
Manufactured by BioLegend
The Anti-TUJ1 mouse IgG primary antibody is a protein that binds specifically to the TUJ1 (Neuron-specific Class III β-Tubulin) antigen. It can be used to detect and identify neuronal cells in various research applications.
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Lab products found in correlation
2 protocols using anti tuj1 mouse igg primary antibody
1
Neuronal Differentiation in Hydrogels
2
Neuronal Differentiation in Hydrogels
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After 7 days in vitro, cell-laden hydrogels were rinsed 3× in PBS for 5 min per rinse and then fixed in 4% paraformaldehyde (Sigma Aldrich) for 5 min at room temperature. After rinsing with PBS, the gels were permeabilized with 0.5% Triton X-100 (Sigma Aldrich), blocked with 5% donkey serum (Invitrogen) and incubated overnight at 4° C with anti-TUJ1 mouse IgG primary antibody (1:1,000, Biolegend). Hydrogels were rinsed in PBS and then incubated for 2 h at room temperature with donkey anti-mouse IgG secondary antibody conjugated to Alexa Fluor 488 (Life Technologies, 1:1,000). Cellular nuclei were counter stained with 0.4 μl/ml DAPI in PBS for 5 min. The labeled hydrogels were examined and imaged using a Zeiss LSM 880 inverted confocal laser scanning microscope.
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