Dimethylaminoethanol

Phosphate-buffered saline
(PBS) solutions were prepared using pre-formulated tablets (Sigma-Aldrich)
in 200 mL of Milli-Q water (>18.2 Ω mean resistivity) to
give [NaCl] = 0.138 M, [KCl] = 0.0027 M, and pH 7.4. Poly(methyl vinyl
ether-alt-maleic anhydride) M_n 80 000 and 311 000 g.mol^–1, dimethylaminoethanol, and N-[4-(aminomethyl)benzyl]rhodamine
6G-amide bis(trifluoroacetate) were purchased from Sigma-Aldrich.
Poly(methyl vinyl ether-alt-maleic anhydride) average M_n 20 000 g mol^–1 was
purchased from Scientific Polymer Products; the dispersity of polymer P2 from the manufacturer is 2.7 and that of P3 is 3.47; the supplier of P1 did not list the M_w value, only the M_n. Sodium chloride was purchased from Fisher Scientific. All solvents
were purchased from VWR or Sigma-Aldrich, and the reagents were used
without further purification unless indicated.

Advanced Dulbecco’s
Modified Eagle’s
Medium, Ham’s F-12K media, and penicillin/streptomycin/amphotericin
B were obtained from Gibco. Poly(ethylene glycol) (M_n 8 kDa) was obtained from MP Biomedicals. Dimethylaminoethanol,
dimethyl sulfoxide, fetal bovine serum, Dulbecco’s phosphate-buffered
saline, poly(ethylene glycol)methyl ether (M_n 20 kDa), and poly(methyl vinyl ether-alt-maleic anhydride) (M_n 80 kDa) were obtained
from Sigma-Aldrich. A live/dead viability/cytotoxicity kit and CellEvent
Caspase-3/7 Green Detection Reagent were obtained from Thermo Fisher.
All solvents were purchased from VWR or Sigma-Aldrich, and reagents
were used without further purification unless indicated.