Ec plan neo fluar 40x

Zeiss microscopes (Thornwood, NY) were used to acquire all images for this study. We used the following systems:
LSM800 inverted confocal on an Axio Observer Z1 stand and equipped with GaAsP detectors and a Zeiss Plan-APOCHROMAT 63x DIC (oil, 1.4NA) objective;
LSM800 upright confocal on an Axio Imager.Z2 stand and equipped with GaAsP detectors and Zeiss Plan-APOCHROMAT 63x DIC (oil, 1.4NA) and Zeiss Plan-APOCHROMAT DIC (UV) VIS-IR 40x (oil, 1.3NA) objectives;
Zeiss Axio Imager.M2 widefield equipped with an AxioCam 506 mono camera and Zeiss Plan-APOCHROMAT 63x DIC (oil, 1.4NA), Zeiss EC Plan NEO FLUAR 40x (oil, 1.3NA) objectives.
All images for localization experiments were taken with a 63x 1.4 NA oil objective; all 0-hour images for injury experiments were taken with a 63x 1.4 NA oil objective; and all 24-hour, 72-hour, and 96-hour post injury images were taken with a 40x 1.3 NA oil objective. Adult v’ada neuron images were taken on 10x and 20x air objectives. All images were processed in FIJI software (https://imagej.net/software/fiji/). Z-stacks and time series images were converted to maximum-intensity projections with the z-project function. Occasionally, clean maximum intensity projections were rendered impossible due to larval movement during imaging; when this was the case, we used the stitching plugin (max intensity option) to generate a complete image.

Zeiss microscopes (Thornwood, NY) were used to acquire images in this study. The following systems were used:

LSM800 inverted confocal on an Axio Observer Z1 stand and equipped with GaAsP detectors and a Zeiss Plan-APOCHROMAT 63x DIC (oil, 1.4NA) objective;

LSM800 upright confocal on an Axio Imager.Z2 stand and equipped with GaAsP detectors and Zeiss Plan-APOCHROMAT 63x DIC (oil, 1.4NA) and Zeiss Plan-APOCHROMAT DIC (UV) VIS-IR 40x (oil, 1.3NA) objectives;

Zeiss Axio Imager.M2 widefield equipped with an AxioCam 506 mono camera and Zeiss Plan-APOCHROMAT 63x DIC (oil, 1.4NA), Zeiss EC Plan NEO FLUAR 40x (oil, 1.3NA) objectives.

Uninjured neurons and 24 hours post dendrotomy (HPD) were imaged with the LSM800 upright with a 40x objective; 4 HPD neurons were imaged with the LSM800 inverted with a 63x objective. Images in Figure 1 were taken using a 40X objective and stitching with up to up to 20×5 tiles. Images in Figures 3 and 4 were taken as 2×2 or 3×3 tiles. For these tiled images, occasionally larvae moved slightly during imaging, so apparent line breaks in representative images are artifacts of movement, not of injury.