Rabbit phospho-p44/42 mitogen-activated protein kinase (MAPK ) (Erk1/2) (Thr202/Tyr204, #4370) and rabbit phospho-p38 MAPK (Thr180/Tyr182, #4631) monoclonal antibodies were purchased from Cell Signaling (Danvers, MA). Mouse anti-chicken glyceraldehyde-3-phosphate dehydrogenase antibody was purchased from Thermo Fisher Scientific (AM4300; Waltham, MA). Alexa Fluor 488 goat anti-rabbit IgG (H + L) secondary antibody was purchased from Invitrogen (A-11008, Carlsbad, CA). Anti-rabbit IgG (H + L) horseradish peroxidase (HRP )-conjugated antibody was purchased from Promega (W4011; Madison, WI). Goat anti-mouse IgG HRP-conjugated antibody (A16078) was purchased from Thermo Fisher Scientific. In addition, HRP-conjugated rabbit anti-6-His antibody (A190-114P) was purchased from Bethyl Laboratories, Inc. (Montgomery, TX) and 4′,6-diamidino-2-phe-nylindole was purchased from Invitrogen (Rockford, IL). Radioimmunoprecipitation assay lysis and extraction buffers were purchased from Thermo Fisher Scientific.
Hrp conjugated rabbit anti 6 his antibody a190 114p
Manufactured by Fortis Life Sciences
Sourced in United States
The HRP-conjugated rabbit anti-6-His antibody (A190-114P) is a laboratory reagent used for the detection and quantification of 6-histidine (6-His) tagged proteins in various applications, such as Western blotting, ELISA, and immunoprecipitation. The antibody is conjugated with horseradish peroxidase (HRP), which enables colorimetric or chemiluminescent detection of the target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Hrp conjugated goat anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions)
Anti rabbit igg h l hrp conjugated antibody, by Promega (1 mentions)
2 protocols using hrp conjugated rabbit anti 6 his antibody a190 114p
1
Phospho-MAPK and GAPDH Antibodies Protocol
2
Immunodetection of Phosphorylated MAPKs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Rabbit phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204, #4370) and rabbit phospho-p38 MAPK (Thr180/Tyr182, #4631) monoclonal antibodies were purchased from Cell Signaling (Danvers, MA, USA). Mouse anti-chicken GAPDH antibody was purchased from Thermo Fisher Scienti c (AM4300; Waltham, MA, USA). Alexa Fluor 488 goat anti-rabbit IgG (H + L) secondary antibody was purchased from Invitrogen (A-11008, Carlsbad, CA, USA). Anti-rabbit IgG (H+L) HRP-conjugated antibody was purchased from Promega (W4011; Madison, WI, USA). Goat anti-mouse IgG HRP-conjugated antibody (A16078) was purchased from Thermo Fisher Scienti c. In addition, HRP-conjugated rabbit anti-6-His antibody (A190-114P) was purchased from Bethyl Laboratories, Inc. (Montgomery, TX, USA) and 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Invitrogen (Rockford, IL, USA). RIPA lysis and extraction buffers were purchased from Thermo Fisher Scienti c.
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