Fitc mouse anti human cd138

Manufactured by BD

Sourced in United States

FITC-Mouse Anti-Human CD138 is a fluorescently labeled antibody that binds to the CD138 antigen, also known as Syndecan-1, which is expressed on the surface of plasma cells. This product is typically used in flow cytometry applications to identify and quantify plasma cells in biological samples.

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Lab products found in correlation

Easysep magnet, by STEMCELL (1 mentions) Lymphoprep, by Axis-Shield (1 mentions) Ms column, by Miltenyi Biotec (1 mentions) Cd138 microbeads, by Miltenyi Biotec (1 mentions) Fetal bovine serum (fbs), by Zhejiang Tianhang Biotechnology (1 mentions) Tnf α, by Novoprotein (1 mentions)

Spelling variants of this product

Anti-CD138 (17 citations) Anti-CD138/FITC (6 citations) CD138-FITC (2 citations) Mouse anti (2 citations) FITC)–conjugated mouse anti-human CD138 (2 citations)

2 protocols using fitc mouse anti human cd138

Plasma Cell Isolation from Bone Marrow

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Bone marrow aspirates from four MM patient samples were obtained at the diagnosis and after chemotherapy. This study was approved by the Institutional Review Board (NCC2016-0279) of the National Cancer Center, Korea.
Bone marrow cells were obtained and separated from heparin-treated blood through density gradient centrifugation (2,200 rpm, 20 min, break 0) using Lymphoprep (Axis-Shield, Oslo, Norway).
Bone marrow plasma cells were enriched using the EasySep Human Whole Blood and Bone Marrow CD138 Positive Selection kit (Stemcell Technologies, Inc., Vancouver, BC, Canada) using EasySep magnets (Stemcell Technologies) or CD138 microbeads (Miltenyi Biotec, Gladbach, Germany) using MS columns (Miltenyi Biotec) according to the manufacturer’s instructions. FITC-Mouse Anti-Human CD138 (BD Pharmingen, San Jose, CA, USA) antibody was used to stain the cells, and the cells were applied directly to flow cytometric analysis using a FACSVerse.

Lee K.J., Lee K.H., Yoon K.A., Sohn J.Y., Lee E., Lee H., Eom H.S, & Kong S.Y. (2017). Chromothripsis in Treatment Resistance in Multiple Myeloma. Genomics & Informatics, 15(3), 87-97.

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Etanercept Modulates BAFF-Induced B-cell Signaling

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Etanercept (Shanghai Sunshine Guojian Pharmaceutical Co., Ltd., China); BAFF (R&D Systems, USA); TNF-α (novoprotein, China); fetal bovine serum (Zhejiang Tianhang Biotechnology, China). TRAF2(C-20), TRAF2-AF647, P-p38 (Santa Cruz Biotechnology, USA); p38 (Abcam, USA); p65 (Cell signaling Technology, USA); P-p65 (affinity Biosciences, USA); FITC Rat Anti-Mouse CD19, APC Mouse Anti-Human CD27, FITC Mouse Anti-Human CD138, PE Rat Anti-Mouse IgM, PE Mouse Anti-Human CD19 (BD Pharmingen, San Diego, CA, USA); TNFRI-APC, IgD-BV421 (BioLegend, San Diego, CA, USA); CD120b (TNF-RII)-APC (Miltenyi Biotec GmbH, Germany); fetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd, China); RPMI-1640 medium (HyClone, USA); Human BAFF ELISA kit, Human TNF-α ELISA kit (Nanjing Fcmacs Biotechnology Co., Ltd, China); Human lymphocyte separation solution, Mice lymphocyte separation solution (Shenzhen Dakco Biotechnology Co., Ltd, China); Cell Counting Kit (CCK-8/WST-8) Cell Proliferation and Activity Assay Kit: Biolite Biotech Trading Partners (Encinitas, CA, USA).

Cai X.Y., Zhu Y., Wang C., Tang X.Y., Han L., Shu J.L., Zhang X.Z., Liang F.Q., Ge J.R., Xu L., Mei D., Zhang L.L, & Wei W. (2020). Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-κB Signaling Pathway in Rheumatoid Arthritis. Frontiers in Pharmacology, 11, 676.

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