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2 protocols using anti actb mouse monoclonal antibody

1

Protein Extraction and Western Blot

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Proteins were extracted from cells or tissues and subjected to 8% Criterion Precast Midi Protein Gel electrophoresis. Regular western procedures were used. Antibodies used for western blotting included anti-LIP rabbit polyclonal (laboratory preparation), anti-HMGB1 rabbit polyclonal (laboratory preparation), eGFP monoclonal antibody (F56-6A1.2.3, Invitrogen), goat anti-rabbit IgG (D111018), anti-ACTB mouse monoclonal antibody (D191047), anti-TP53 rabbit polyclonal antibody (D220082), anti-ACSL3 rabbit polyclonal antibody (D261226), and anti-ACSL4 rabbit polyclonal antibody (D121771). Unlabeled antibodies were purchased from Sangon Biotech Co., Ltd. (Shanghai, China).
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2

Western Blot Analysis of Cellular Proteins

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Proteins were extracted from cells or tissues and subjected to 8% Criterion Precast Midi Protein Gel electrophoresis. Regular western procedures were used. Antibodies used for western blotting included anti-LIP rabbit polyclonal (laboratory preparation), anti-HMGB1 rabbit polyclonal (laboratory preparation), eGFP monoclonal antibody (F56-6A1.2.3, Invitrogen), goat anti-rabbit IgG (D111018), anti-ACTB mouse monoclonal antibody (D191047), anti-TP53 rabbit polyclonal antibody (D220082), anti-ACSL3 rabbit polyclonal antibody (D261226), and anti-ACSL4 rabbit polyclonal antibody (D121771). Unlabeled antibodies were purchased from Sangon Biotech Co., Ltd. (Shanghai, China).
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