Dexamethasone sodium phosphate was from ValleyVet.com . Rapamycin (dissolved in DMSO first) and FTY720 (fingolimod HCL; dissolved in DMSO first) were from Selleckchem.com . DQ ovalbumin, sodium heparin, ACK lysing buffer, Ficoll-Paque Plus, Freund’s complete adjuvant (“FCA”), and Freund’s incomplete adjuvant (“FIA”) were from ThermoFisher. Hen ovalbumin (“OVA”), hen egg lysozyme (“HEL”), and monophosphoryl lipid A (“MPLA”; dissolved in DMSO first) were from Sigma-Aldrich. Protease- and Ig-free BSA was from SouthernBiotech. Antibodies for flow cytometry were from Biolegend, eBioscience, and Jackson ImmunoResearch Laboratories. Cell counting beads for flow cytometry were from Bangs Laboratories. Mouse Th1/Th2/Th17 CBA kit was from BD Biosciences. Clodronate liposomes and control liposomes were a gift from Roche Diagnostics GmbH. Mouse CD4+ T cell isolation kit (negative selection), mouse CD4+CD25+ regulatory T cell isolation kit, and mouse CD3ε MicroBead kit were from Miltenyi Biotec. eFluor 670 was from eBioscience (dissolved in DMSO first). The peptide OVA323–339 was from AnaSpec.
Clodronate liposomes
Manufactured by Roche
Clodronate liposomes are a laboratory product used for cell depletion studies. They are composed of the bisphosphonate compound clodronate encapsulated within lipid vesicles. Clodronate liposomes are designed to be taken up by phagocytic cells, leading to their depletion.
Automatically generated - may contain errors
Lab products found in correlation
Ack lysing buffer, by Thermo Fisher Scientific (2 mentions)
Sodium heparin, by Thermo Fisher Scientific (1 mentions)
Mouse th1 th2 th17 cba kit, by BD (1 mentions)
Efluor 670, by Thermo Fisher Scientific (1 mentions)
Rapamycin, by Selleck Chemicals (1 mentions)
Freund s incomplete adjuvant, by Thermo Fisher Scientific (1 mentions)
Fty720, by Selleck Chemicals (1 mentions)
Ficoll paque plus, by Thermo Fisher Scientific (1 mentions)
Mouse cd3ε microbead kit, by Miltenyi Biotec (1 mentions)
Mouse cd4 cd25 regulatory t cell isolation kit, by Miltenyi Biotec (1 mentions)
Dq ovalbumin, by Thermo Fisher Scientific (1 mentions)
Cilengitide, by MedChemExpress (1 mentions)
2 protocols using clodronate liposomes
1
Murine T Cell Isolation and Stimulation
2
Macrophage Depletion with Clodronate Liposomes
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Clodronate liposomes were purchased from Roche and prepared as described previously (Van Rooijen et al., 1994 (link)). Clodronate liposomes have been used extensively to specifically deplete macrophages in vivo by inducing apoptosis in these cells without affecting other cell types. Cilengitide was obtained from Med Chem Express (NJ, United States).
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