Mice were pretreated with 40 μg/mouse i.v. of β-glucan, or dextran. 1 hour later, rabbit anti-chicken egg albumin IgG (60 μg/30 μL, Sigma-Aldrich, C6534) or PBS alone were injected subcutaneously in the dorsal skin of wild-type mice, followed immediately by the intravenous injection of ovalbumin (400 μg/mouse, Sigma-Aldrich). After 4 hours, the skin containing the injection site was removed from euthanized mice. For analysis of edema, the solution of chicken egg albumin contained 0.15% Evans blue dye (Sigma-Aldrich) and measurements were conducted as described (Utomo et al., 2008 (link)). For immunostaining of neutrophils, the injected site of skin was excised. 10 μm OCT-embedded, frozen sections were fixed in ice-cold acetone, blocked with Dako protein block solution (DAKO), and incubated with Rat anti-Ly6G Ab (1:100, Biolegend, 127602), Alexa488 conjugated anti-Rat IgG (1:500, Invitrogen, A21208) and DAPI (1:500). Ly6G-positive neutrophils per HPF were counted and averaged.
Rat anti ly6g ab
Manufactured by BioLegend
Rat anti-Ly6G Ab is a monoclonal antibody that specifically binds to the Ly6G antigen, which is expressed on the surface of granulocytic myeloid cells, particularly neutrophils, in the rat. The antibody can be used for the identification and characterization of these cell types.
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Lab products found in correlation
2 protocols using rat anti ly6g ab
1
Edema and Neutrophil Assessment in Mice
2
Edema and Neutrophil Assessment in Mice
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Mice were pretreated with 40 μg/mouse i.v. of β-glucan, or dextran. 1 hour later, rabbit anti-chicken egg albumin IgG (60 μg/30 μL, Sigma-Aldrich, C6534) or PBS alone were injected subcutaneously in the dorsal skin of wild-type mice, followed immediately by the intravenous injection of ovalbumin (400 μg/mouse, Sigma-Aldrich). After 4 hours, the skin containing the injection site was removed from euthanized mice. For analysis of edema, the solution of chicken egg albumin contained 0.15% Evans blue dye (Sigma-Aldrich) and measurements were conducted as described (Utomo et al., 2008 (link)). For immunostaining of neutrophils, the injected site of skin was excised. 10 μm OCT-embedded, frozen sections were fixed in ice-cold acetone, blocked with Dako protein block solution (DAKO), and incubated with Rat anti-Ly6G Ab (1:100, Biolegend, 127602), Alexa488 conjugated anti-Rat IgG (1:500, Invitrogen, A21208) and DAPI (1:500). Ly6G-positive neutrophils per HPF were counted and averaged.
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