The changes in mitochondrial MMP were measured with an MMP assay kit with JC-1 (Solarbio, cat # M8650, Beijing, China) according to the manufacturer’s instructions. Briefly, treated N2A cells were harvested and washed with cold PBS. Then, the cells were suspended in a mixture of 1 mL of culture medium and 1 mL of JC-1 staining working solution (10 μg/mL) for 20 min in the dark at 37 °C. Subsequently, the cells were washed with cold staining buffer three times. Afterwards, cells were counted in a BD Accuri C6 flow cytometer (BD Bioscience, San Jose, CA, USA).
Mmp assay kit with jc 1
Manufactured by Solarbio
Sourced in China
The MMP Assay Kit with JC-1 is a laboratory tool designed to measure the activity of Matrix Metalloproteinases (MMPs) in biological samples. The kit utilizes the fluorescent dye JC-1 to detect and quantify MMP activity. This product is intended for research use only and does not include any information or claims about its intended use or applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mmp assay kit with jc 1
1
Measuring Mitochondrial Membrane Potential
2
Mitochondrial Membrane Potential Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded in confocal plates and treated accordingly. Mitochondrial membrane potential (MMP) was evaluated by MMP Assay Kit with JC-1 (Solarbio, Beijing, China) according to the manufacturer's instructions. When the MMP is high, JC-1 can produce red fluorescence. When it is weak, JC-1 generates green fluorescence. It is quite convenient to detect the change of MMP by the change of fluorescence color. The relative ratio of red to green fluorescence is often used to assess mitochondrial depolarization. All images were obtained with a confocal microscope.
3
Mitochondrial Membrane Potential Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MMP was detected with the MMP Assay Kit with JC-1 (Solarbio, Beijing, China). After cultured in 6-well plates under the different experimental conditions for 48 h, HK-2 cells were washed in PBS and incubated with JC-1 dye for 20 min at 37°C. After the dye was removed by washing, the cells were immediately imaged using a TCS SP8 confocal microscope (Leica Microsystems, Wetzlar, Germany). JC-1 monomers emit a green fluorescence, while aggregates of JC-1 show intense red fluorescence. In apoptotic cells, JC-1 does not form aggregates in mitochondria with low MMP and exhibit green fluorescence.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!