Et gfp band pass filter cube

The GCaMP3 fluorescence is quantified via the ΔF/F ratio. ΔF/F = (F–F₀)/F₀, where F is the GCaMP3 fluorescence of a given time point during touch while F₀ is the averaged based line in the ROIs in the first 2 min before touch treatment. The GCaMP3 fluorescence calculation for the leaf was performed in each selected leaf position for every ten seconds, while in the trichomes for every second. Video recordings were made with a 1.5× objective on an SMZ18 stereomicroscope (Nikon Instruments Europe BV, Amsterdam, Netherlands) equipped with an ORCA-Flash4.0 (C11440) camera (Hamamatsu, Solothurn, Switzerland) and eGFP emission/excitation filter set (AHF Analysentechnik AG, Tübingen, Germany). Light was supplied to the stereomicroscope using fiber optics. Video with a resolution of 512 × 512 pixels was acquired using NIS-Elements software (Nikon) with 1 frame s⁻¹ frequency. Video recordings for Figs. 5c–f, 6a, b, supplemental Figs. 5–7, 8e, f, and 9 were made with a 1x objective (total magnification ×0.77) on a M205FA stereomicroscope (Leica Microsystems BV Amsterdam, the Netherlands) and ET GFP band pass filter cube (Leica). Videos with a resolution of 1920 × 1440 pixels were acquired using LAS X (Leica) with 1 frame s^-1 frequency. Recordings were carried out in the dark at 22 °C.

The GCaMP3 fluorescence is quantified via the ΔF/F ratio. ΔF/F = (F–F₀)/F₀, where F is the GCaMP3 fluorescence of a given time point during touch while F₀ is the averaged based line in the ROIs in the first 2 min before touch treatment. The GCaMP3 fluorescence calculation for the leaf was performed in each selected leaf position for every ten seconds, while in the trichomes for every second. Video recordings were made with a 1.5× objective on an SMZ18 stereomicroscope (Nikon Instruments Europe BV, Amsterdam, Netherlands) equipped with an ORCA-Flash4.0 (C11440) camera (Hamamatsu, Solothurn, Switzerland) and eGFP emission/excitation filter set (AHF Analysentechnik AG, Tübingen, Germany). Light was supplied to the stereomicroscope using fiber optics. Video with a resolution of 512 × 512 pixels was acquired using NIS-Elements software (Nikon) with 1 frame s⁻¹ frequency. Video recordings for Figs. 5c–f, 6a, b, supplemental Figs. 5–7, 8e, f, and 9 were made with a 1x objective (total magnification ×0.77) on a M205FA stereomicroscope (Leica Microsystems BV Amsterdam, the Netherlands) and ET GFP band pass filter cube (Leica). Videos with a resolution of 1920 × 1440 pixels were acquired using LAS X (Leica) with 1 frame s^-1 frequency. Recordings were carried out in the dark at 22 °C.