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Anti human mitochondria clone 113 1

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Anti-human mitochondria (clone 113–1) is a laboratory reagent used for the detection and analysis of mitochondria in human cells. It is a monoclonal antibody that specifically binds to a mitochondrial protein, allowing researchers to visualize and study the structure and function of mitochondria in human samples.

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Lab products found in correlation

Anti human cd45 clone hi30, by BD (2 mentions) Anti mouse cd45 clone 30 f11, by BD (2 mentions) Anti ki67 clone 8d5, by Cell Signaling Technology (2 mentions) Alexa fluor 568 goat anti rabbit igg h l, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 goat anti mouse igg h l, by Thermo Fisher Scientific (2 mentions)

2 protocols using anti human mitochondria clone 113 1

1

Immunofluorescence Staining of Cells

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Cells were fixed, permeabilized, blocked against nonspecific adhesion, immunostained for target proteins, and then imaged on an inverted Eclipse Ti epifluorescence microscope (Nikon). Primary antibodies were administered at manufacturer recommended concentration: anti-human CD45 (clone HI30, Becton Dickinson), anti-mouse CD45 (clone 30-F11, Becton Dickinson), anti-human mitochondria (clone 113–1, MilliporeSigma), anti-Ki67 (clone 8D5, Cell Signaling Technology), and anti-phospho-histone H2A.X (Ser139, clone 20E3, Cell Signaling Technology). The secondary antibodies used were Alexa Fluor 488 goat anti-mouse IgG (H+L) (Invitrogen) and Alexa Fluor 568 goat anti-rabbit IgG (H+L) (Invitrogen).
Yankaskas C.L., Thompson K.N., Paul C.D., Vitolo M.I., Mistriotis P., Mahendra A., Bajpai V.K., Shea D.J., Manto K.M., Chai A.C., Varadarajan N., Kontrogianni-Konstantopoulos A., Martin S.S, & Konstantopoulos K. (2019). A microfluidic assay for the quantification of the metastatic propensity of breast-cancer specimens. Nature biomedical engineering, 3(6), 452-465.
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2

Immunofluorescence Staining of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed, permeabilized, blocked against nonspecific adhesion, immunostained for target proteins, and then imaged on an inverted Eclipse Ti epifluorescence microscope (Nikon). Primary antibodies were administered at manufacturer recommended concentration: anti-human CD45 (clone HI30, Becton Dickinson), anti-mouse CD45 (clone 30-F11, Becton Dickinson), anti-human mitochondria (clone 113–1, MilliporeSigma), anti-Ki67 (clone 8D5, Cell Signaling Technology), and anti-phospho-histone H2A.X (Ser139, clone 20E3, Cell Signaling Technology). The secondary antibodies used were Alexa Fluor 488 goat anti-mouse IgG (H+L) (Invitrogen) and Alexa Fluor 568 goat anti-rabbit IgG (H+L) (Invitrogen).
Yankaskas C.L., Thompson K.N., Paul C.D., Vitolo M.I., Mistriotis P., Mahendra A., Bajpai V.K., Shea D.J., Manto K.M., Chai A.C., Varadarajan N., Kontrogianni-Konstantopoulos A., Martin S.S, & Konstantopoulos K. (2019). A microfluidic assay for the quantification of the metastatic propensity of breast-cancer specimens. Nature biomedical engineering, 3(6), 452-465.
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