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Dox hcl

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DOX·HCl is a chemical compound commonly used in research and laboratory settings. It is the hydrochloride salt of doxylamine, a sedating antihistamine. DOX·HCl is a white, crystalline powder that is soluble in water and various organic solvents. The product's core function is to serve as a research tool, without interpretation of its intended use.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (7 mentions) Dulbecco s modified eagle s medium dmem, by Merck Group (5 mentions) Trypsin edta solution, by Merck Group (4 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (4 mentions) 4 cyano 4, by Merck Group (3 mentions) 4 nitrophenyl chloroformate, by Thermo Fisher Scientific (2 mentions) 2 azobis isobutyronitrile aibn, by Merck Group (2 mentions) Hoechst 33342, by Merck Group (2 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (2 mentions) Trizol lysis reagent, by Thermo Fisher Scientific (2 mentions) Quantitect reverse transcription kit, by Qiagen (2 mentions) Methacrylate oegma, by Merck Group (2 mentions) Vinylbenzyl chloride, by Merck Group (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Valspodar, by Merck Group (1 mentions) N methyl 2 pyrrolidinone nmp, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Wst 1, by Roche (1 mentions) Pluronic p85, by BASF (1 mentions)

9 protocols using dox hcl

1

PLGA-Based Dox Delivery System

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Poly(DL-lactic-co-glycolic) (PLGA, acid-capped, 75:25, MW 28.8 kDa, inherent viscosity 0.28 dL/g) was obtained from Evonik Corp (Parsippany, NJ). N-methyl-2-pyrrolidinone (NMP) and Valspodar were obtained from Sigma–Aldrich (St. Louis, Missouri). Dox HCl was obtained from LC Laboratories (Woburn, MA). Pluronic P85 were obtained from BASF (Ludwigshafen, Germany). RPMI-1640, fetal bovine serum, and penicillin-streptomycin were obtained from ThermoFisher Scientific (Waltham, MA). WST-1 was obtained from Roche Applied Sciences (Penzberg, Germany). All items were used as received.
Jeganathan S., Budziszewski E., Hernandez C., Dhingra A, & Exner A.A. (2020). Improving Treatment Efficacy of In Situ Forming Implants via Concurrent Delivery of Chemotherapeutic and Chemosensitizer. Scientific Reports, 10, 6587.
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2

Sunflower Polymer Prodrug for Doxorubicin

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A 50 mL falcon tube equipped with a magnetic stirrer was charged with the polymer precursor P((HEMA-sf-OEGMA-folate)-st-(GMA-hydrazide)) (180.0 mg, 29.2 μmol of hydrazide), doxorubicin hydrochloride (Dox•HCl, LC Laboratories, Woburn, MA) (33.8 mg, 58.4 μmol), a drop of acetic acid, and anhydrous methanol (6 mL). The tube was sealed, and the reaction mixture was placed in the dark at room temperature for 48 h. The resultant sunflower polymeric prodrug, P((HEMA-sf-OEGMA-folate)-st-(GMA-Dox)), was purified by extensive dialysis against anhydrous methanol (2 L) for 24 h, followed by dialysis against distilled water (4 L) for another 24 h, and collected by freeze-drying. Yield: 78.7% (155 mg). The amount of Dox conjugated to the sunflower polymer was determined by absorbance at 480 nm based on a standard curve obtained from free Dox•HCl in water.
Wang C.E., Wei H., Tan N., Boydston A.J, & Pun S.H. (2015). Sunflower Polymers for Folate-Mediated Drug Delivery. Biomacromolecules, 17(1), 69-75.
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3

Synthesis and Characterization of Multivalent Cysteine Conjugates

Cited 1 time
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DOX·HCl was purchased from LC Laboratories. 4-Nitrophenyl chloroformate was purchased from Acros. Dulbecco's modified Eagle's medium (DMEM), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Triton X-100 and Hoechst 33342 were purchased from Sigma-Aldrich. Penicillin-streptomycin solution and fetal bovine serum (FBS) were purchased from Invitrogen. 2-Azobis(isobutyronitrile) (AIBN, Sigma-Aldrich) was purified by recrystallization in anhydrous ethanol22 (link). N,N′-(t-butyoxycarbonyl)cystamine (MBC)23 (link) and POEG macroCTA24 (link) were prepared according to the literature. All other reagents were of analytical or chromatographic grade.
Sun J.J., Chen Y.C., Huang Y.X., Zhao W.C., Liu Y.H., Venkataramanan R., Lu B.F, & Li S. (2017). Programmable co-delivery of the immune checkpoint inhibitor NLG919 and chemotherapeutic doxorubicin via a redox-responsive immunostimulatory polymeric prodrug carrier. Acta Pharmacologica Sinica, 38(6), 823-834.
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4

Synthesis and Characterization of Drug-Loaded Polymeric Nanoparticles

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5-ASA was purchased from Frontier Scientific (UT, U.S.A). DOX·HCl was purchased from LC Laboratories (MA, U.S.A). 4-Cyano-4 [(dodecylsulfanylthiocarbonyl)sulfanyl] pentanoic acid, 2-Azobis-(isobutyronitrile) (AIBN), poly(ethylene glycol) methacrylate (average Mn = 950, PEG950), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Dulbecco's Modified Eagle's Medium (DMEM) and folic acid were purchased from Sigma-Aldrich (MO, U.S.A). AIBN was purified by recrystallization in anhydrous ethanol. N-Succinimidyl methacrylate was purchased from TCI (U.S.A). RPMI-1640 medium and folate-deficient RPMI-1640 medium, fetal bovine serum (FBS) and penicillin-streptomycin solution were purchased from Invitrogen (NY, U.S.A). All solvents used in this study were of HPLC grade.
Huang H., Huang Y., Chen Y., Luo Z., Zhang Z., Sun R., Wan Z., Sun J., Lu B., Zhang L., Hu J, & Li S. (2021). A novel immunochemotherapy based on targeting of cyclooxygenase and induction of immunogenic cell death. Biomaterials, 270, 120708.
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5

Polymer-based Nanocarrier Synthesis and Characterization

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DOX·HCl was purchased from LC Laboratories (MA, U.S.A). Creatine, 4-Cyano-4 [(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid, vinylbenzyl chloride (VBC) monomer, and oligo(ethylene glycol) methacrylate (average Mn = 500, OEG500), 2-Azobis(isobutyronitrile) (AIBN), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and Dulbecco’s Modified Eagle’s Medium (DMEM) were purchased from Sigma-Aldrich (MO, U.S.A). AIBN was purified by recrystallization in anhydrous ethanol. Opti-MEM medium, fetal bovine serum (FBS), Dulbecco’s phosphate buffered saline (DPBS), penicillin-streptomycin solution and TRIzol lysis reagent were purchased from Invitrogen (NY, U.S.A.). QuantiTect Reverse Transcription Kit was purchased from Qiagen (MD, U.S.A). All solvents used in this study were HPLC grade.
Xu J., Sun J., Ho P.Y., Luo Z., Ma W., Zhao W., Rathod S.B., Fernandez C.A., Venkataramanan R., Xie W., Yu A.M, & Li S. (2019). Creatine Based Polymer for Codelivery of Bioengineered MicroRNA and Chemodrugs against Breast Cancer Lung Metastasis. Biomaterials, 210, 25-40.
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6

Synthesis and Characterization of Polymeric Nanocarriers

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PPMP·HCl was synthesized and purified following a published patent (WO 2005/049827A2). Methacryl chloride,triethylamine,4-Cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl] pentanoic acid, oligo(ethylene glycol) methacrylate OEGMA (average Mn = 500), 2,2-Azobis (isobutyronitrile)(AIBN), Dulbecco’s Modified Eagle’s Medium (DMEM), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (MO, U.S.A).DOX·HCl was purchased from LC Laboratories (MA, U.S.A). PTX was purchased from AK Scientific Inc. (CA, U.S.A.). Fetal bovine serum (FBS), penicillin-streptomycin solution and TRIzol lysis reagent were purchased from Invitrogen (NY, U.S.A.). QuantiTect Reverse Transcription Kit was purchased from Qiagen (MD, U.S.A).
Xu J., Zhao W., Sun J., Huang Y., Wang P., Venkataramanan R., Yang D., Ma X., Rana A, & Li S. (2018). Novel glucosylceramide synthase inhibitor based prodrug copolymer micelles for delivery of anticancer agents. Journal of controlled release : official journal of the Controlled Release Society, 288, 212-226.
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7

Polymer-based Anticancer Drug Delivery

Cited 1 time
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Vinylbenzyl chloride, 2, 2-Azobis(isobutyronitrile) (AIBN), 1,4-dioxane, trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and Dulbecco’s Modified Eagle’s Medium (DMEM) were all purchased from Sigma-Aldrich (St. Louis, MO, United States). AIBN was purified by recrystallization in anhydrous ethanol. DOX⋅HCl and DTX were purchased from LC Laboratories (Woburn, MA, United States). PTX was purchased from AK Scientific Inc. (Union City, CA, United States). Fetal bovine serum (FBS) and penicillin-streptomycin solution were all purchased from Invitrogen (NY, United States). POEG macroCTA were prepared as previously reported (Sun et al., 2016a (link)).
Li Z., Sun J., Huang Y., Liu Y., Xu J., Chen Y., Liang L., Li J., Liao Q., Li S, & Zhou K. (2018). A Nanomicellar Prodrug Carrier Based on Ibuprofen-Conjugated Polymer for Co-delivery of Doxorubicin. Frontiers in Pharmacology, 9, 781.
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8

Synthesis and Characterization of Polymeric Nanoparticles

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FTS was synthesized and purified following a published literature [18 (link)]. Vinylbenzyl chloride, 4-Cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid, oligo(ethylene glycol) methacrylate OEGMA (average Mn=500), 2, 2-Azobis(isobutyronitrile) (AIBN), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and Dulbecco’s Modified Eagle’s Medium (DMEM) were all bought from Sigma-Aldrich (MO, U. S. A.). AIBN was purified by recrystallization in anhydrous ethanol. Paclitaxel was purchased from AK Scientific Inc. (CA, U. S. A.). DOX·HCl was purchased from LC Laboratories (MA, USA). Fetal bovine serum (FBS) and penicillin-streptomycin solution were purchased from Invitrogen (NY, U. S. A.). Cell culture and animals were similarly handled as described before [32 (link)].
Sun J., Chen Y., Li K., Huang Y., Fu X., Zhang X., Zhao W., Wei Y., Xu L., Zhang P., Venkataramanan R, & Li S. (2016). A Prodrug Micellar Carrier Assembled from Polymers with Pendant Farnesyl Thiosalicylic Acid Moieties for Improved Delivery of Paclitaxel. Acta biomaterialia, 43, 282-291.
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9

Dasatinib-Loaded Polymer Nanoparticles

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Dasatinib and DOX·HCl were purchased from LC Laboratories. 4-nitrophenyl chloroformate was purchased from Acros. 2-Azobis(isobutyronitrile) (AIBN) was purchased from Sigma-Aldrich and purified by recrystallization in anhydrous ethanol. Dulbecco’s phosphate buffered saline (DPBS) was purchased from Lonza. Penicillin-streptomycin solution and fetal bovine serum (FBS) were purchased from Invitrogen. Dulbecco’s Modified Eagle’s Medium (DMEM), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Triton X-100, Hoechst 33342 and LysoTracker Green DND-26 were all purchased from Sigma-Aldrich. N-methacryloyl-N-(tert-butoxycarbonyl)aminohexyl methacrylamide (MBA) [39 (link)], N-methacryloyl-N′-(t-butyoxycarbonyl)cystamine (MBC) [40 (link)], POEG macroCTA [34 (link)], and 4-nitrophenyl-actived Dasatinib Das-NO2 [41 (link)] were prepared according to the literatures. All other reagents were of analytical or chromatographic grade.
Sun J., Liu Y., Chen Y., Zhao W., Zhai Q., Rathod S., Huang Y., Tang S., Kwon Y.T., Fernandez C., Venkataramanan R, & Li S. (2017). Doxorubicin Delivered by A Redox-responsive Dasatinib-containing Polymeric Prodrug Carrier for Combination Therapy. Journal of controlled release : official journal of the Controlled Release Society, 258, 43-55.
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