Lanosterol

Posaconazole (PSZ) was obtained from Carbosynth (Compton, UK). Fluconazole (FLU) was purchased from Cayman Chemical (Ann Arbor, MI). 5α-Cholesta-8,24-dien-3β-ol (zymosterol), 14-demethyl-14-dehydroLanosterol (FF-MAS), 4,4-dimethylcholesta-8,24-dien-3β-ol (T-MAS), cholesta-7,24-dienol, 7-dehydrodesmosterol, and Cholesterol-d7 were purchased from Avanti Polar Lipids Inc. (Alabaster, AL). Ergosterol was acquired from Acros Organics (Geel, Belgium). M199 medium, squalene, hexane, and fetal bovine serum (FBS) were acquired from Sigma-Aldrich Corporation (St. Louis, MO). Lanosterol was obtained from American Radiolabeled Chemicals Inc. (St. Louis, MO). Episterol was purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). Cholesterol was ordered from MP Biomedicals LLC (Irvine, CA). Optima LC/MS-grade water, optima-grade methanol, 2-propanol, formic acid, adenine, 10 N sodium hydroxide solution, and HEPES buffer (N-2-hydroxyethylpoperazine-N′-ethanesulfonic acid, pH 7.4) were bought from Fisher Scientific (Pittsburgh, PA). Hemin and DMSO were obtained from Alfa Aesar (Ward Hill, MA). Phosphate-buffer-saline (PBS) 10X was acquired from Mediatech Inc (Manassas, VA). Pen Strep and Schneider's Drosophila media were bought from Life Technologies Corporation (Grand Island, NY). Brain Heart Infusion (BHI) was purchased from Becton-Dickinson and Company (Sparks, MD).