All DNA oligonucleotides used in this study were synthesized and purified with high performance liquid chromatography (HPLC) by Bioneer® (Daejeon, Korea). The sequence information of oligonucleotides employed in this study is in Table S1 . The personal glucose meter (PGM) whose dynamic range for glucose detection is from 0.6 to 33 mM was purchased from Accu-Chek (Roche, Basel, Switzerland). Cerium (IV) oxide nanoparticle (CeO2 NP), sodium acetate, glucose, ethidium bromide (EtBr), hydroethidine, and 2,5-dihydroxybenzoic acid (DHB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The deoxynucleoside triphosphate (dNTP) and i-TaqTM DNA polymerase were purchased from Intron Biotechnology Inc. (Daejeon, Korea). Ultrapure DNase/RNase-free distilled water (DW) purchased from Bioneer® was used in all experiments. All chemicals used in this study were of analytical grade.
I taqtm dna polymerase
Manufactured by iNtRON Biotechnology
I-TaqTM DNA polymerase is a thermostable DNA polymerase enzyme used for DNA amplification in the polymerase chain reaction (PCR) process. It exhibits high thermal stability and efficient DNA synthesis activity.
Automatically generated - may contain errors
Lab products found in correlation
Hydroethidine, by Merck Group (1 mentions)
2 5 dihydroxybenzoic acid dhb, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Ultrapure dnase rnase free distilled water, by Bioneer (1 mentions)
Glucose, by Merck Group (1 mentions)
Accu chek, by Roche (1 mentions)
Maxime pcr premix kit i taq, by iNtRON Biotechnology (1 mentions)
2 protocols using i taqtm dna polymerase
1
Glucose Detection via CeO2 Nanoparticles
2
Amplification of Tams1 gene in T. annulata
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Two primer pairs (N516: 5′-GTAACCTTTAAAAACGT and N517: 5′-GTTACGAACATGGGTTT) were used to amplify a 721 bp fragment from the gene encoding 30-KDa major T. annulata merozoite surface antigens (Tams 1) according to d'Oliveira method [19] .
PCR was performed using 25 μl volume as follows: 12 μl of H2O, 2 μl of each primer, 4 μl of genomic DNA and 5 μl of Maxime PCR PreMix Kit (i-Taq) (iNtRON Biotechnology, Korea) containing 1x reaction buffer (10x), 2.5 U of i-TaqTM DNA Polymerase (5U/ μl), 2.5 mM of each dNTPs and 1x Gel loading buffer. The amplification was performed with an initial denaturation at 94°C for 5 min followed by 35 cycles of 94°C for 3 min, 55°C for 1 min, 72°C for 2 min and final extension step at 72°C for 10 min. PCR products were separated on 1.5% agarose gel.
PCR was performed using 25 μl volume as follows: 12 μl of H2O, 2 μl of each primer, 4 μl of genomic DNA and 5 μl of Maxime PCR PreMix Kit (i-Taq) (iNtRON Biotechnology, Korea) containing 1x reaction buffer (10x), 2.5 U of i-TaqTM DNA Polymerase (5U/ μl), 2.5 mM of each dNTPs and 1x Gel loading buffer. The amplification was performed with an initial denaturation at 94°C for 5 min followed by 35 cycles of 94°C for 3 min, 55°C for 1 min, 72°C for 2 min and final extension step at 72°C for 10 min. PCR products were separated on 1.5% agarose gel.
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