Human fibrinogen

All oligonucleotides at high performance liquid chromatography (HPLC) grade were purchased from Eurofins Genomics (Japan) (Table S1). Before use, each 150 μM aptamer in PBS was annealed by heating at 95°C for 3 min using a dry-bath incubator (Major Science, Taiwan) and cooling immediately in a heat block previously stored at 25°C. The annealed aptamer was diluted in PBS for the desired concentrations. All solutions were prepared using ultrapure water from a Milli-Q water purification system (Merck Millipore, USA). Human fibrinogen, mouse serum, 2-amino-2-hydroxymethyl-1,3-propanediol; Tris, EDTA-2Na-2H₂O, 40 w/v% acrylamide/bis mixed solution 19:1, ammonium persulfate (APS), N,N,N,N′-tetramethylethylene; TEMED, 10w/v% polyoxyethylene(20) sorbitan monolaurate solution; 10% Tween 20, Dulbecco’s PBS (−), urea, and protamine sulfate from salmon were purchased from FUJIFILM Wako Pure Chemicals Industries (Japan). Normal human serum and normal mouse plasma were purchased from Cosmo Bio (Japan). Human thrombin and bivalirudin trifluoroacetate salt were purchased from Sigma-Aldrich (MO, USA). Mouse thrombin (Creative Biomart, NY, USA), argatroban monohydrate (TCI, Japan), normal human plasma (George King Bio-Medical, KS, USA), and 10 mM sodium acetate (pH 5.5; Cytiva, MA, USA) were used as received. C57BL/6 mice were purchased from Japan SLC (Shizuoka, Japan).