Serum autoantibodies were determined by using mouse-specific ELISA kits for antinuclear antibodies (ANA), anti-dsDNA antibodies (Alpha Diagnostic Int’l, San Antonio, TX) and anti-smooth muscle antibodies (ASMA) (Cusabio LLC, Houston, TX), respectively. Serum lipocalin-2 levels were measured by mouse lipocalin-2/NGAL quantikine ELISA kit (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions. MDA-protein adducts in the liver and colon tissues were analyzed according to our earlier published methods (Wang et al., 2008 (link); Wang et al., 2012a (link); Wang et al., 2012b (link)). Serum cytokines were determined using Cytokine 17-Plex Mouse ProcartaPlex Panel (Invitrogen, Carlsbad, CA) by following the manufacturer’s instructions.
Anti dsdna antibodies
Manufactured by Alpha Diagnostic
Anti-dsDNA antibodies are a type of autoantibody that binds to double-stranded DNA (dsDNA). They are commonly used in the diagnosis and monitoring of systemic lupus erythematosus (SLE), an autoimmune disorder.
Automatically generated - may contain errors
2 protocols using anti dsdna antibodies
1
Autoantibodies and Oxidative Stress Markers
2
Serum Biomarker Profiling Protocol
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Sera were assessed for anti-nuclear antibodies (ANA; Alpha Diagnostic International, San Antonio, TX), anti-dsDNA antibodies (Alpha Diagnostic Int’l), blood urea nitrogen (BUN; Arbor Assays, Ann Arbor, MI), and serum creatinine (Arbor Assays) per manufacturers’ protocols. For ANA and anti-dsDNA assays, sera were measured in duplicate at a 1:100 dilution in a 96-well plate format, and the HRP-coupled secondary Ab was goat anti mouse IgG (H and L). Negative and positive control sera, as well as 5 point calibration curve samples, provided by the manufacturer, were run concurrently with the unknown samples. Sera were diluted 1:10 for BUN assays and 1:30 for creatinine assays, per manufacturers’ protocols. Sera were run in duplicate alongside a 5 (creatinine) or 7 (BUN) point standard curve. All assays were read at 450 nm using an Emax Plus Reader (Molecular Devices). Unknowns were compared with a calibration curve containing five dilution points on each assay plate. In all cases, the coefficient of determination for the standard curve (r2) was ≥0.98.
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