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Brilliant violet 421 conjugated streptavidin

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Brilliant Violet 421-conjugated streptavidin is a fluorescently labeled protein that binds to biotin with high affinity. It can be used in various immunoassays and cell-based applications to detect biotinylated targets.

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Lab products found in correlation

Pd l1 10f 9g2, by BioLegend (1 mentions) Pd l2 ty25, by Thermo Fisher Scientific (1 mentions) Foxp3 transcription factor staining buffer kit, by Cytek Biosciences (1 mentions) Adenosine, by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Recombinant human m csf, by Thermo Fisher Scientific (1 mentions) Adenosine 5 triphosphate disodium salt atp, by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Cd39 inhibitor pom1, by Bio-Techne (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) Il 10, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Recombinant human gm csf, by Novartis (1 mentions) Interleukin 4 (il 4), by Novartis (1 mentions)

Spelling variants of this product

Brilliant Violet 421 (66 citations) Streptavidin-Brilliant Violet 421 (29 citations) Brilliant Violet 421™ Streptavidin-conjugated Abs (2 citations)

4 protocols using brilliant violet 421 conjugated streptavidin

1

Multiparameter Flow Cytometry Analysis

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Cultured cells and primary T cells were stained with the indicated Abs. Data were obtained with Gallios (Beckman Coulter) and analyzed using FlowJo (Tree Star). Abs against Bcl6 (K112-91) and CXCR5 (2G8) were purchased from BD bioscience. Abs against CD3 (145-2C11), CD80 (16-10A-1), CD86 (GL-1), and PD-L2 (TY25) were purchased from eBioscience. The Ab against CD8 (5H10) was purchased from Thermo Fisher Scientific. Abs against CD3 (17A2), CD4 (RM4-5), CD28 (37.5), CD45 (30-F11), ICOS (C398.4A), ICOSL (HK5.3), PD-1 (RMP1-30), and PD-L1 (10F.9G2), Brilliant violet 421-conjugated streptavidin, armenian hamster IgG (HTK888), rat IgG2b (RTK4530), and syrian hamster IgG (SHG-1) were purchased from Biolegend. FoxP3 Transcription Factor Staining Buffer Kit (TONBO) was used for the staining of Bcl6.
Mizuno R., Sugiura D., Shimizu K., Maruhashi T., Watada M., Okazaki I.M, & Okazaki T. (2019). PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation. Frontiers in Immunology, 10, 630.
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2

Antigen Presentation Assay for ILC2s

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A plasmid encoding the EαGFP fusion protein, a kind gift of Dr. Marc K. Jenkins, was used to generate the EαGFP protein. To assess whether wt and C3ar1−/− ILC2s can present processed antigen differently, we cultured sorted lung ILC2s (ICOS+IL-33R+) in the presence of 100 μg/ml EαGFP protein for 16h at 37°C, 5% CO2. Cells were incubated with a biotin-conjugated antibody (1:100) specific to the Eα52–68 bound to MHC (I-Ab) (clone eBio YAe-eBiosciences) for 20 mins at RT, washed, then followed by Brilliant Violet 421-conjugated streptavidin (BioLegend). As negative control, cells were incubated with EαGFP, where the YAe antibody was omitted, but Brilliant Violet 421-conjugated streptavidin was added.
Gour N., Smole U., Yong H.M., Lewkowich I.P., Yao N., Singh A., Gabrielson E., Wills-Karp M, & Lajoie S. (2018). C3a is required for ILC2 function in allergic airway inflammation. Mucosal immunology, 11(6), 1653-1662.
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3

Antigen Presentation Assay for ILC2s

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A plasmid encoding the EαGFP fusion protein, a kind gift of Dr. Marc K. Jenkins, was used to generate the EαGFP protein. To assess whether wt and C3ar1−/− ILC2s can present processed antigen differently, we cultured sorted lung ILC2s (ICOS+IL-33R+) in the presence of 100 μg/ml EαGFP protein for 16h at 37°C, 5% CO2. Cells were incubated with a biotin-conjugated antibody (1:100) specific to the Eα52–68 bound to MHC (I-Ab) (clone eBio YAe-eBiosciences) for 20 mins at RT, washed, then followed by Brilliant Violet 421-conjugated streptavidin (BioLegend). As negative control, cells were incubated with EαGFP, where the YAe antibody was omitted, but Brilliant Violet 421-conjugated streptavidin was added.
Gour N., Smole U., Yong H.M., Lewkowich I.P., Yao N., Singh A., Gabrielson E., Wills-Karp M, & Lajoie S. (2018). C3a is required for ILC2 function in allergic airway inflammation. Mucosal immunology, 11(6), 1653-1662.
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4

Macrophage Activation Assay Protocol

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LPS (Escherichia coli serotype O55:B5) and adenosine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Deuterated adenosine was from CDN Isotopes (Quebec, Canada). adenosine 5′-triphosphate disodium salt (ATP) was from Thermo Fisher Scientific (Waltham, MA, USA). Recombinant human M-CSF, IFNγ, and IL-10 were obtained from Peprotech (Rocky Hill, NJ, USA). Recombinant human GM-CSF and IL-4 were from Novartis AG (Basel, Switzerland). The RPMI 1640 medium, l-glutamine, streptomycin, penicillin, and heat-inactivated fetal calf serum (FCS) were obtained from Gibco, Thermo Fisher Scientific. CD39 inhibitor POM-1 was from Tocris Bioscience (Bristol, UK). The cell proliferation dye CFSE and calcium sensor Fluo-4, AM was from Molecular Probes, Thermo Fisher Scientific. Brilliant Violet 421-conjugated streptavidin used as a second step in flow cytometry analyses was purchased from BioLegend (San Diego, CA, USA). Phorbol 12-myristate 13-acetate (PMA), ionomycin calcium salt (ionomycin) from S. conglobatus and monensin A sodium salt (monensin) were purchased from Sigma-Aldrich.
Ohradanova-Repic A., Machacek C., Charvet C., Lager F., Le Roux D., Platzer R., Leksa V., Mitulovic G., Burkard T.R., Zlabinger G.J., Fischer M.B., Feuillet V., Renault G., Blüml S., Benko M., Suchanek M., Huppa J.B., Matsuyama T., Cavaco-Paulo A., Bismuth G, & Stockinger H. (2018). Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Frontiers in Immunology, 9, 852.
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