Jurkat cells (2 × 106) were transfected in Nucleofector solution (Nucleofector Kit V, program X-01; Amaxa Biosystems) with 2 μM of nonsense siRNA (Qiagen), Bid siRNA (Qiagen) or Bim siRNA (Qiagen) using the Nucleofector apparatus. MT-2 cells were transfected in Nucleofector solution (Nucleofector Kit V, program O-17; Amaxa Biosystems) with 2 μM of nonsense siRNA (Qiagen) or HIF-1α siRNA (Qiagen). Seventy-two hours after transfection, cells were collected for protein expression analysis and different treatments as indicated in the figure legends.
Nonsense sirna
Manufactured by Qiagen
Nonsense siRNA is a laboratory tool used to control for off-target effects in RNA interference (RNAi) experiments. It is a synthetic small interfering RNA (siRNA) that does not target any known gene, serving as a negative control to assess the specificity of RNAi-mediated gene silencing.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using nonsense sirna
1
Transfection of Jurkat and MT-2 Cells
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