Sepharose cl 4b chromatography media

Doxorubicin hydrochloride (DOX·HCl), folate, dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), cholesterol, DMSO, (NH₄)₂SO₄, CHCl₃, Sepharose CL-4B chromatography media and folate-free RPMI 1640 cell culture medium were purchased from Sigma-Aldrich Chemical Co (St. Louis, MO, USA). DMEM high glucose medium was purchased from the Hyclone of Thermo Scientific (IL, USA). Trypsin was purchased from Beyotime Institute of Biotechnology (Beijing, China). 1, 2-Dioleoyl-3-Trimethylammonium-Propane (Chloride Salt) (DOTAP), monomethoxy polyethylene glycol 2000-distearoyl phosphatidylethanolamine (mPEG-DSPE) and PEG-bis-amine (3350 Da) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). PD-10 desalting columns were purchased from GE Healthcare Biosciences (PA, USA). All reagents and solvents were of analytical or HPLC grade and were used without further purification. FAM or Cy5-labeled Bmi1 siRNA, and Chol-modified Bmi1 siRNA (sense strand: 5'-CCA GAC CAC UAC UGA AUA UAA-3'; antisense strand: 5'-UUA UAU UCA GUA GUG GUC UGG UU-3') were synthesized by Ribobio Co., Ltd (Guangzhou, China).

Doxorubicin hydrochloride (Dox·HCl), folic acid, dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), Chol, L-glutamate, calcein, Sepharose CL-4B chromatography media and folate-free Roswell Park Memorial Institute (RPMI) 1640 cell culture medium were purchased from Sigma-Aldrich Chemical Co., (St Louis, MO, USA). High glucose Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from the Hyclone of Thermo Fisher Scientific (Waltham, MA, USA). Trypsin was purchased from Beyotime Institute of Biotechnology (Wuhan, People’s Republic of China). Hydrogenated soybean phosphatidylcholine (HSPC), monomethoxy polyethylene glycol 2000-distearoyl phosphatidylethanolamine (mPEG-DSPE) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). PD-10 desalting columns were purchased from GE Healthcare Bio-Sciences Corp., (Piscataway, NJ, USA). All reagents and solvents were of analytical or high-performance liquid chromatography grade and were used without further purification.