81 inverted microscope

Roots of two-week-old Arabidopsis lines expressing MVLG_06175ΔSP-mCherry, MVLG_06175-mCherry, and mCherry alone, leaves of four- or six-week-old Arabidopsis line expressing MVLG_06175ΔSP-mCherry, MVLG_06175-mCherry, MVLG_05122ΔSP-CFP, mCherry alone, and CFP alone, and their corresponding WT controls were observed by confocal microscopy. Images were acquired by an Olympus Fluoview FV-1000 confocal coupled to an Olympus 1 × 81 inverted microscope ((Olympus, East Syracuse, NY, USA), a PlanApoN 60× objective, and FV-10 ASW 2.1 software. A single channel scanning configuration was set up for the acquisition of mCherry (excitation 587 nm, emission 610 nm) and CFP (excitation 458 nm, emission 476 nm) using a 543 nm HeNe laser and a 458 line of argon laser, respectively. Scanning was set at a speed of 2 μs/pixel to acquire z-stacks of each visual field. Images are presented as either single plane images or stacked images.