In our experiments, pentobarbital sodium (Sanofi, France), N-(2-hydroxyethyl) piperazine-N′-(2-ethanesulfonic acid) (HEPES) (Sigma Aldrich, Germany), NaCl (Merck, Germany), KCl (Merck), D-glucose (Merck), NaHCO3 (Merck), KH2PO4 (Scharlau Chemie SA, Spain), CaCl2·2H2O (Merck), MgSO4·7H2O (Fluka AG, Germany), collagenase from Clostridium histolyticum type IV (Sigma Aldrich), albumin, bovine serum fraction V, minimum 98% (Sigma Aldrich), ethylene glycol tetraacetic acid (Sigma Aldrich), 2-thiobarbituric acid (TBA) (4,6-dihydroxypyrimidine-2-thiol) (Sigma Aldrich), trichloroacetic acid (TCA) (Valerus, Bulgaria), 6-hydroxydopamine (Merck), 2,2′-dinitro-5,5′-dithiodibenzoic acid (DTNB) (Merck), lactate dehydrogenase (LDH) kit (Randox, UK), D(+) sucrose (Fluka, Germany), NaH2PO4 (Merck), MgCl2·6H2O, Percoll (Sigma Aldrich), (3-[4,5-dimethylthiazol -2-yl]-2, 5diphenyl-tetrazolium bromide) (Sigma Aldrich), dimethyl sulfoxide (DMSO) (Valerus, Bulgaria) were used.
Lactate dehydrogenase ldh kit
Manufactured by Randox
Sourced in United Kingdom
The Lactate dehydrogenase (LDH) kit is a laboratory diagnostic tool used to measure the activity of the LDH enzyme in biological samples. LDH is an important enzyme involved in the conversion of lactate to pyruvate during the anaerobic metabolism of glucose. The kit provides a quantitative analysis of LDH levels, which can be used as an indicator of various medical conditions.
Automatically generated - may contain errors
Lab products found in correlation
Sodium pentobarbital, by Sanofi (4 mentions)
2 2 dinitro 5 5 dithiodibenzoic acid dtnb, by Merck Group (4 mentions)
D glucose, by Merck Group (4 mentions)
Sodium chloride (nacl), by Merck Group (4 mentions)
Potassium chloride (kcl), by Merck Group (4 mentions)
Nahco3, by Merck Group (4 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (4 mentions)
Collagenase from clostridium histolyticum type 4, by Merck Group (3 mentions)
Cacl2 2h2o, by Merck Group (3 mentions)
2 thiobarbituric acid, by Merck Group (3 mentions)
T buooh, by Merck Group (2 mentions)
Bovine serum fraction 5, by Merck Group (2 mentions)
Hepes, by Merck Group (2 mentions)
N 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes, by Merck Group (1 mentions)
Mgcl2 6h2o, by Merck Group (1 mentions)
Percoll, by Merck Group (1 mentions)
Nah2po4, by Merck Group (1 mentions)
Feso4, by Merck Group (1 mentions)
Cacl2 x 2 h2o, by Merck Group (1 mentions)
4 protocols using lactate dehydrogenase ldh kit
1
Neuronal Cell Isolation and Characterization
2
Liver Oxidative Stress Evaluation
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The chemicals used in the experiments were: Pentobarbital sodium (Sanofi, France), (N-[2-Hydroxyethyl]piperazine-N’-[ethanesulfonic acid]) (HEPES) (Sigma-Aldrich, Germany), NaCl (Merck, Germany), KCl (Merck), D-glucose (Merck), NaHCO3 (Merck), KH2PO4 (Scharlau Chemie SA, Spain), CaCl2.2H2 O (Merck), MgSO4.7H2 O (Fluka AG, Germany), collagenase from Clostridium histolyticum type IV (Sigma-Aldrich), albumin, bovine serum fraction V, minimum 98% (Sigma-Aldrich), EGTA (Sigma-Aldrich), 2-thiobarbituric acid (4,6-dihydroxypyrimidine-2-thiol; TBA) (Sigma-Aldrich), trichloroacetic acid (TCA) (Valerus, Bulgaria), 2,2’- dinitro-5,5’- dithiodibenzoic acid (DTNB) (Merck), lactate dehydrogenase (LDH) kit (Randox, UK), t-BuOOH (Sigma-Aldrich), and CCl4 (Merck).
3
Quantification of Flavonoid Standards
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The standards of flavonoids were purchased from Extrasynthese (Genay, France) except for kaempferid (Fluka, Buchs, Germany). HPLC-grade solvents and analytical-grade chemicals NaCl, KCl, NaHCO3, CaCl2.2H2O, FeSO4, D-glucose, 2,2’-dinitro-5,5’-dithiodibenzoic acid (DTNB) and CCl4 were provided by Merck (Darmstadt, Germany). HEPES, EGTA, albumin, bovine serum fraction V, minimum 98%, 2-thiobarbituric acid (4,6-dihydroxypyrimidine-2-thiol; TBA), and t-BuOOH were provided by Sigma-Aldrich (Germany). In our experiments, pentobarbital sodium (Sanofi, France), KH2PO4 (Scharlau Chemie SA, Spain), MgSO4.7H2O (Fluka AG, Germany), trichloroacetic acid (TCA), ascorbinic acid and glycerol (Valerus, Bulgaria), and lactate dehydrogenase (LDH) kit (Randox, UK) were used.
4
Biochemical Analysis of Tissue Samples
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The following chemicals and reagents, including pentobarbital sodium (Sanofi, France), HEPES (Sigma Aldrich, Germany), NaCl (Merck, Germany), KCl (Merck), D-glucose (Merck), NaHCO3 (Merck), KH2PO4 (ScharlauChemie SA, Spain), K2HPO4 (ScharlauChemie SA, Spain), glycerol (Scharlau-Chemie SA, Spain), CaCl2x2H2O (Merck), MgSO4x 7H2O (Fluka AG, Germany), collagenase from Clostridium histolyticum type IV (Sigma Aldrich), albumin, bovine serum fraction V, minimum 98% (Sigma Aldrich), EGTA (Sigma Aldrich), 2-thiobarbituric acid (4,6-dihydroxypyrimidine-2-thiol; TBA) (Sigma Aldrich), trichloroacetic acid (TCA) (Valerus, Bulgaria), 2,2'-dinitro-5,5'-dithiodibenzoicacid (DTNB) (Merck), lactate dehydrogenase (LDH) kit (Randox, UK) were used.
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