Bs 20 gjm

Manufactured by Biosharp

Sourced in China

The BS-20-GJM is a compact and versatile laboratory centrifuge. It is designed for general-purpose applications, capable of handling a variety of sample types and volumes. The centrifuge features a maximum rotor speed of 6,000 rpm and a maximum RCF of 4,000 x g, making it suitable for a range of sample preparation and separation tasks.

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Lab products found in correlation

Dcfh da probe, by Beyotime (1 mentions) Hoechst 33342, by Beyotime (1 mentions) Anti rabbit igg h l alexa fluor 555, by Abcam (1 mentions) Anti cd24, by Abcam (1 mentions) Antifade mounting medium with dapi, by Beyotime (1 mentions) Tcs sp8 confocal laser scanning microscope, by Leica (1 mentions) Pm150211, by Procell (1 mentions) Fibronectin f2006, by Merck Group (1 mentions) Lsm 880, by Zeiss (1 mentions) Hoechst 33342 p0133, by Beyotime (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

6 protocols using bs 20 gjm

DCFH-DA Oxidation Imaging Assay

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Cells were seeded into live imaging culture dishes (Biosharp, BS-20-GJM, China) before adding the diluted DCFH-DA probe (1:1000, Beyotime, S0033S, China) along with Hoechst 33342 (500×, Beyotime, C1027, China) in culture medium at 37°C. The cells were subsequently incubated for 20 min, washed cells three times with 37°C medium before capturing images with a Leica DMi8 (Germany) confocal microscope using the 488-nm laser line.

Li H., Ren J., Cui H., Wang D., Zhao R., Liu X., Tian S., Wang J., Zhang J., Li P., Thorne R.F, & Duan S. (2023). Dexamethasone Induces Senescence-Associated Changes in Trabecular Meshwork Cells by Increasing ROS Levels Via the TGFβ/Smad3-NOX4 Axis. Cell Transplantation, 32, 09636897231177356.

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CD24 Expression in OC Cell Lines

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OC cell lines were cultured in confocal dishes (Cat No. BS-20-GJM, Biosharp, China) and then fixed with 4% paraformaldehyde in PBS for 15 min. The cells were permeabilized with 0.5% Triton X-100 in PBS for 10 min and then incubated in 5% BSA for 1 hr. The cells were then incubated overnight at 4 °C with anti-CD24 (1:100; Cat No. ab202073, Abcam, UK). The secondary antibody anti-rabbit IgG (H + L) Alexa Fluor 555 (Cat No. ab150074, Abcam, UK) was used at a 1:500 dilution for 1 h. Antifade mounting medium with DAPI (Cat No. C1002, Beyotime, China) was used for nuclear staining. Then, the cells were imaged on a Leica confocal microscope.

Liu J., Yan C, & Xu S. (2024). LncRNA IL21-AS1 facilitates tumour progression by enhancing CD24-induced phagocytosis inhibition and tumorigenesis in ovarian cancer. Cell Death & Disease, 15(5), 313.

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Live-cell Imaging of Transfected Proteins

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For live-cell imaging, 35-mm glass-bottom dishes (BS-20-GJM, Biosharp, China) were coated with 10 μg/mL fibronectin (F2006; Sigma, MO, USA) in PBS for at least 3 h at 37°C, washed with PBS twice, and immersed in complete DMEM without phenol red (PM150211; Procell, China) before seeding of cells. The time-lapse images of cells with transient transfection of hABHD16A-GFP and hIFITM1-DsRed were acquired with Leica TCS SP8 laser scanning confocal microscope. Appropriate filters, a heated sample environment (37°C), controlled 5% CO₂, and a ×60/1.5 oil objective were used. The recording was set as every 10 s for 360 s, and one focal plane was recorded for all live cell videos.

Shi X., Li X., Xu Z., Shen L., Ding Y., Chen S., Mao L., Liu W, & Xu J. (2022). ABHD16A Negatively Regulates the Palmitoylation and Antiviral Function of IFITM Proteins. mBio, 13(6), e02289-22.

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Quantifying PD-L1 Expression on Cell Surface and Cytoplasm

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Cells were cultured on a confocal dish (cat. BS-20-GJM, Biosharp, Tallin, Estonia) and washed twice with pre-warmed PBS. For cytoplasmic membrane PD-L1 staining, cells were fixed with 5% PFA (Paraformaldehyde) for 15 min, followed by 0.5% triton (PFA + Triton X-100) for 10 min. For cell membrane PD-L1 staining, cells were fixed with 5% PFA for 15 min only. Cells were blocked with 1% BSA and then incubated with the PD-L1 antibody for 1 h at room temperature. Primary antibody: Anti-human PD-L1 (ABCAM, cat. ab213524). Secondary Antibody: Anti-Rabbit Alexa Fluor 568 (ABCAM, cat. Ab175470). For immunofluorescence, images were acquired using a fluorescence microscope (Olympos, Tokyo, Japan; CKX3) and analysed by Image J. For immunofluorescence colocalisation, images were acquired using a confocal microscope (Zeiss; LSM 880) and analysed by ZEN software.

Zhang H., Zhou S.J., Shen C.F., Zhou Y.N., Wu C.Y., Zhu M.Y., Yu Q.M., Awadasseid A., Wu Y.L, & Zhang W. (2023). PD-L1 dimerisation induced by biphenyl derivatives mediates anti-breast cancer activity via the non-immune PD-L1–AKT–mTOR/Bcl2 pathway. Journal of Enzyme Inhibition and Medicinal Chemistry, 38(1), 2230388.

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Immunofluorescence Analysis of TFEB and ABCA2

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For immunofluorescence studies, 5×10⁴ cells/well were seeded on the confocal dish (BS-20-GJM; Biosharp, Hefei, China). After 48 h, cells were treated and fixed with 3.7% paraformaldehyde, washed with PBS, and permeabilized with Triton X-100 for a half-hour, followed by incubation blocking solution (1% BSA) for 1 h. Cells were incubated with the following antibodies diluted in PBS with 1% BSA overnight at 4°C: anti-TFEB (dilution 1:500) and anti-ABCA2 (dilution 1:100). The cells were washed with PBS and incubated with rhodamine (TRITC) goat anti-rabbit IgG (H+L) (AS040, ABclonal, Wuhan, China) for 1 h at room temperature. Cell nuclei were stained with Hoechst 33342 (P0133, Beyotime Biotechnology, Shanghai, China). Labeled cells were examined under the ZESIS LSM880 microscope with a ×100/1.4 objective lens. Confocal microscopy images were acquired and processed with LSM880 system confocal microscope software.

Zhu X., Zhuo Y., Wu S., Chen Y., Ye J., Deng Y., Feng Y., Liu R., Cai S., Zou Z., Wang B., Wu C.L., Zeng G, & Zhong W. (2021). TFEB Promotes Prostate Cancer Progression via Regulating ABCA2-Dependent Lysosomal Biogenesis. Frontiers in Oncology, 11, 632524.

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MEHP-Induced Mitochondrial Dynamics in HTR-8 Cells

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HTR-8 cells were inoculated with 6 × 10⁵ per well in 35 mm confocal dishes (Biosharp, BS-20-GJM) before MEHP treatment. MitoTracker (Maokangbio, M7514) and Hoechst 33342 (Invitrogen, H3570) were used to label mitochondria and nuclei respectively. Live cells were visualized 24 h post the MEHP treatment by confocal microscopy (LEICA, THUNDER Imager 3D Live Cell).

Zhao S., Hong Y., Liang Y.Y., Li X.L., Shen J.C., Sun C.C., Chu L.L., Hu J., Wang H., Xu D.X., Zhang S.C., Xu D.D., Xu T, & Zhao L.L. (2022). Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast. Redox Biology, 55, 102414.

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