Lipids in cells were extracted using 1.5 mL/well cell lipid extracting solution on the ice for 1 h. The solution was dried using nitrogen and then dissolved in 80 μL of lipid lysis solution for TG quantification by triacylglycerol assay kit (NANJINGJIANCHENG Bio, China). Subsequently, the cellular protein was extracted with 0.2 mL/well of RIPA lysis buffer on ice for 20 min and measured by BCA protein assay kit (Dingguochangsheng, China). Liver tissue was homogenized with liver lipid extraction (mass (g): volume (mL) = 1:9) and the supernatant was obtained by centrifugation at 3000 rpm for 10 min at 4 °C. The supernatant was dried by nitrogen and then residue was dissolved in 3 mL lipid lysis solution for lipid quantification with triacylglycerol assay kit and total cholesterol assay kit (NANJINGJIANCHENG Bio, China). The result is expressed as micromoles of lipid in per gram of cellular protein or per gram of liver weight.
Triacylglycerol assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Triacylglycerol Assay kit is a laboratory tool designed for the quantitative determination of triacylglycerol levels in various sample types. It provides a standardized and reliable method for the analysis of this important lipid class.
Automatically generated - may contain errors
Lab products found in correlation
Total cholesterol assay kit, by Nanjing Jiancheng (6 mentions)
Low density lipoprotein cholesterol assay kit, by Nanjing Jiancheng (4 mentions)
Enzymatic biochemical kits, by Nanjing Jiancheng (3 mentions)
High density lipoprotein cholesterol assay kit, by Nanjing Jiancheng (3 mentions)
Bca protein assay kit, by Dingguo (1 mentions)
Rat il 1β elisa kit, by Cusabio (1 mentions)
Alanine aminotransferase assay kit, by Nanjing Jiancheng (1 mentions)
Aspartate aminotransferase assay kit, by Nanjing Jiancheng (1 mentions)
6 protocols using triacylglycerol assay kit
1
Quantification of Cellular and Hepatic Lipids
2
Serum Lipid Profile in Mice
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Peripheral blood of mice were collected from the posterior orbital venous plexus. The blood was centrifuged for 10 min under the rotate speed of 3000 rpm and the serum was collected for the analysis of serum lipids. The detection is performed according to the speci cation of enzymatic biochemical kits (Nanjing Jiancheng) via microplate reader (TECAN): Total Cholesterol Assay kit (Cat A111-1), Triacylglycerol Assay kit (Cat A110-1), Low Density Lipoprotein Cholesterol Assay kit (Cat A113-1), and High Density Lipoprotein Cholesterol Assay kit (Cat A112-1). The level of non-HDL-C was calculated by HDL-C subtracted from TC content.
3
Enzymatic Lipid Profiling Protocol
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Enzymatic biochemical kits (Nanjing Jiancheng) were used to determine serum lipids via microplate reader (TECAN): Total Cholesterol Assay kit (Cat A111-1), Triacylglycerol Assay kit (Cat A110-1), Low Density Lipoprotein Cholesterol Assay kit (Cat A113-1), and High Density Lipoprotein Cholesterol (Cat A112-1).
4
Serum Lipid Profile in Mice
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Peripheral blood of mice were collected from the posterior orbital venous plexus. The blood was centrifuged for 10min under the rotate speed of 3000rpm and the serum was collected for the analysis of serum lipids. The detection is performed according to the speci cation of enzymatic biochemical kits (Nanjing Jiancheng) via microplate reader (TECAN): Total Cholesterol Assay kit (Cat A111-1), Triacylglycerol Assay kit (Cat A110-1), Low Density Lipoprotein Cholesterol Assay kit (Cat A113-1), and High Density Lipoprotein Cholesterol Assay kit (Cat A112-1). The level of non-HDL-C was calculated by HDL-C subtracted from TC content.
5
Serum Lipid and Enzyme Analysis
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After blood was set at room temperature for 30 min, serum was separated by centrifuging at 3500 rpm for 20 min at 4 °C. Serum TG and TC contents were measured by triacylglycerol assay kit, total cholesterol assay kit (NANJINGJIANCHENG Bio, China), transaminase levels were detected using alanine aminotransferase assay kit and aspartate aminotransferase assay kit (NANJINGJIANCHENG Bio, China), and inflammatory factors levels were measured using Rat IL-1β ELISA kit (CUSABIO, China), Rat IL-4 ELISA kit and Rat TNF-α ELISA kit (ExCELL Bio, China).
6
Murine Serum Lipid Analysis
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Peripheral blood of mice were collected from the posterior orbital venous plexus. The blood was centrifuged for 10 min under the rotate speed of 3000 rpm and the serum was collected for the analysis of serum lipids. The detection is performed according to the specification of enzymatic biochemical kits (Nanjing Jiancheng) via microplate reader (TECAN): Total Cholesterol Assay kit (Cat A111–1), Triacylglycerol Assay kit (Cat A110–1), Low Density Lipoprotein Cholesterol Assay kit (Cat A113–1), and High Density Lipoprotein Cholesterol Assay kit (Cat A112–1). The level of non-HDL-C was calculated by HDL-C subtracted from TC content.
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