Cobas c 513 analyzer

Blood samples were collected from the antecubital vein after at least a 10-h fast for HbA1c and fasting glucose (mg/dL) levels during the health checkup. HbA1c levels were measured using a turbidimetric inhibition immunoassay with a Cobas c513 analyzer (Roche Diagnostics, Tokyo, Japan), with a reference value <5.7%, following the International Federation of Clinical Chemistry methods, the Diabetes Control and Complications Trial standards, and the National Glycohemoglobin Standardization Program. The intra-assay coefficient of variation was 2.3%, and the inter-assay coefficient of variation was 2.4% during the study period. Serum glucose level was measured using a Cobas Integra 800 apparatus (Roche Diagnostics, Basel, Switzerland), using the hexokinase method. HbA1c collected at study inclusion was used for the analysis. The Kangbuk Samsung Hospital’s Laboratory Medicine Department in Seoul, Korea, has been accredited by the Korean Association of Quality Assurance for Clinical Laboratories and the Korean Society of Laboratory Medicine. In addition, the laboratory participated in the College of American Pathologists’ Survey and Proficiency Testing program.

Glucose, TC, TAG, HDL-C, LDL-C, HbA₁c, and IL-6 were analyzed by routine hospital methods in the Laboratory of Analysis of the Complejo Hospitalario of Jaén. Particularly, glucose, TC, TAG, and HDL-C were measured by a spectrophotometric procedure using a Cobas c analyzer (Roche/Hitachi). LDL-C levels were estimated indirectly with the Friedewald equation. The percentage of HbA₁c was determined by a turbidimetric inhibition immunoassay (TINIA) of hemolyzed blood samples on a Cobas c-513 analyzer (Roche). IL-6 was quantified by an electrochemiluminescence immunoassay on a Cobas e-801 analyzer (Roche).