Hcx pl apo cs 40x 1

Time-lapse microscopy imaging was performed on a Zeiss AxioObserver Z1 microscope (Carl Zeiss, Germany) with a Plan-Apochromat 40X/1.3 (NA = 1.3, working distance = 0.21 mm) or Plan-Apochromat 63X/1.4 (NA = 1.4, working distance = 0.19mm) oil lens objective, a camera (AxioCam MRm; Carl Zeiss), and Apotome (ApoTome 2; Carl Zeiss). z-stack images were taken with AxioVision module Z-stack (Zeiss). Three-dimensional reconstruction of z-stacks and two-dimensional projections were conducted using the Imaris 7.1 Software (Bitplane Scientific Software, Belfast, UK) and the Z Projection ImageJ plugin, respectively, 35. For confocal reflection microscopy, tumor-spheres were seeded on glass bottom 35mm-μ-dishes, incubated for different times and subsequently fixed with 4% formalin. Z-stack images were acquired using a Leica TCS-SP2 AOBS confocal microscopy with a HCX PL APO CS 40x/1.25 oil lens objective and a 488 nm Argon laser in reflection at room temperature.

Time-lapse microscopy imaging was performed on a Zeiss AxioObserver Z1 microscope (Carl Zeiss, Germany) with a Plan-Apochromat 40X/1.3 (NA = 1.3, working distance = 0.21 mm) or Plan-Apochromat 63X/1.4 (NA = 1.4, working distance = 0.19mm) oil lens objective, a camera (AxioCam MRm; Carl Zeiss), and Apotome (ApoTome 2; Carl Zeiss). z-stack images were taken with AxioVision module Z-stack (Zeiss). Three-dimensional reconstruction of z-stacks and two-dimensional projections were conducted using the Imaris 7.1 Software (Bitplane Scientific Software) and the ZProjection ImageJ plugin, respectively. Mosaic images were taken using AxioVision software (Carl Zeiss). For confocal reflection microscopy, tumorospheres were seeded on glass bottom 35mm-μ-dishes and fixed with 4% formalin. Z-stack images were acquired using a Leica TCS-SP2 AOBS confocal microscopy with a HCX PL APO CS 40x/1.25 oil lens objective and a 488 nm Argon laser in reflection at room temperature.