Bicalutamide casodex

Sunitinib malate (Sigma-Aldrich, St Louis, MO, USA), was resuspended in DMSO to a final concentration of 10 mM and stored at +4°C, according to the manufacturer’s instructions. Sorafenib (Bayer Pharmaceuticals, Leverkusen, Germany) was resuspended in DMSO to a stock concentration of 10 mM and stored at -20°C. Bicalutamide (Casodex) (Sigma-Aldrich, St Louis, MO, USA), was resuspended in DMSO to a stock concentration of 10 mM, according to the manufacturer’s instructions. Drugs were diluted into the culture medium shortly before performing the assays.

Antibodies for biochemical studies were as follows from Cell Signaling Technology: anti-p-EGFR Tyr 1068 (catalog 2234), anti-p-Akt/PKB (catalog 9271), anti-Akt/PKB (total) (catalog 9272). Anti-p-ERK1/2 Tyr 204 (catalog sc7383), anti-ERK2 (total) (catalog sc154), anti-p53 (catalog sc126), anti-p21 (catalog sc397), anti-p27 (catalog sc528), anti-EGFR antibody for IP (A-10, catalog sc373746), anti-AR (catalog N20) was from Santa Cruz Biotechnology. Anti-cyclin D1 (catalog 33-3500) was from Invitrogen. Anti-EGFR for WB (catalog 06-847) was from Millipore. Anti-p-Ser15-p53 (catalog MAB1839) was from R&D Systems. Anti-ERβ (catalog 06-629) was from Upstate. Anti-tubulin was from Sigma-Aldrich. The tyrosine kinase inhibitor of the epidermal growth factor receptor (EGFR), ZD 1839, was from Selleckchem. Bisphenol A (BPA) was from Sigma-Aldrich. The antiandrogen bicalutamide (Casodex) was from Sigma-Aldrich. The antiestrogen ICI 182,780 was from Astra-Zeneca. The MEK-1 inhibitor PD 98,059 was from Sigma-Aldrich. All other reagents were of chemical grade.