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2 2 5 5 tetramethyl 3 pyrroline 3 carboxamide

Manufactured by Merck Group
Sourced in United States

2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide is a chemical compound used in laboratory settings. It serves as a building block for the synthesis of various organic compounds. The molecule contains a pyrroline ring structure with two methyl groups on the 2 and 5 positions, as well as a carboxamide functional group.

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2 protocols using 2 2 5 5 tetramethyl 3 pyrroline 3 carboxamide

1

Radical Scavenging Assay Reagents

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5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) and bis-(2-hydroxyethyl)-iminotris-(hydroxymethyl)-methane (Bis-Tris) were purchased from Dojindo Laboratories, Ltd. (Kumamoto, Japan). Dimethyl sulfoxide (DMSO) and 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Manganese (IV) oxide (MnO2: >70%) was purchased from Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan). 4-Hydroxy-2,2,6,6-tetramethyl piperidine-1-oxyl (TEMPOL) and 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide (TPC) were purchased from Sigma-Aldrich Corp. (St. Louis, MO). Ammonia solution (28–30%), H2O2 (30.0–35.0%), and glutathione (GSH) were purchased from Wako Pure Chemical Industries, Ltd. (Fujifilm Wako Pure Chemical Corp., Osaka, Japan). Cerium nitrate hexahydrate was purchased from Nikki Corp., Saitama, Japan. Deionized water (Milli-Q system; Merck Millipore, Billerica, MA) was used for all experiments.
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2

Antioxidant Evaluation of Leucoselect Compounds

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GSE (Leucoselect) was obtained from Indena (Milan, Italy). Di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) was purchased from Tokyo Chemical Industry (Tokyo, Japan). 5,5-Dimethyl-1-pyrroline N-oxide (DMPO), and xanthine oxidase (XOD) were from Labotec (Tokyo, Japan). 2,2,5,5-Tetramethyl-3-pyrroline-3-carboxamide (TPC), 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), hypoxanthine (HPX), superoxide dismutase (SOD; from bovine erythrocytes), and allopurinol were obtained from Sigma-Aldrich (Saint Louis, MO, USA). 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Tx), rose bengal, and sodium azide (NaN3) were purchased from Wako Pure Chemical Industries (Osaka, Japan). All other reagents used were of analytical grade.
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