Antibodies were obtained from several sources: anti-O-GlcNAc (RL-2, #MA1-072) from Pierce Biotechnology (Rockford, IL); anti-MAN1A1 (#M-3694) from Sigma-Aldrich (St. Louis, MO); anti-pAkt (ser-473, #9271s), anti-Akt, anti-pFOXO3 (ser-294, #5538s), and anti-pFOXO3 (thr-32, #9464s) from Cell Signaling (Danvers, MA); anti-Erk (K-23, #sc-94), anti-pErk (E-4, #sc-7383), anti-pIkk (T23, #sc-101706), and anti-Ikk (H470, #sc-7607) from Santa Cruz Biotechnology; anti-FOXO3 (#07-702) and anti-GAPDH (#MAB-374) from Merck Millipore (Billerica, MA). Akt inhibitor, MK-2206 dihydrochloride (#sc-364537) was from Santa Cruz Biotechnology (Santa Cruz, CA) and Erk inhibitor, PD98059 (#9900) was obtained from Cell Signaling (Danvers, MA). PNGase-F (#P0704) was purchased from New England Biolabs (Ipswich, MA), Pisum Sativum Agglutinin (PSA, #L-1050), biotinylated PSA (#B-1055), biotinylated Concanavalin A (#B-1005) were obtained from Vector Laboratories (Burlingame, CA). Mannosidase I inhibitor, kifunensine (#K1140), OGA inhibitor, [O-(2-Acetamido-2-deoxy-D-glucopyranosylidenamino)-N-phenylcarbamate; PUGNAc, #A7229], and cycloheximide (CHX, #C104450) were purchased from Sigma-Aldrich.
Anti man1a1 m
Manufactured by Merck Group
The Anti-MAN1A1 (#M is a laboratory reagent used for the detection and analysis of the MAN1A1 protein. It is a specific antibody that binds to the MAN1A1 protein, allowing researchers to identify and quantify its presence in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Kifunensine, by Merck Group (2 mentions)
Pugnac, by Merck Group (2 mentions)
Anti gapdh mab374, by Merck Group (2 mentions)
Pd98059, by Cell Signaling Technology (2 mentions)
Anti pfoxo3 thr 32, by Cell Signaling Technology (2 mentions)
Cycloheximide (chx), by Merck Group (2 mentions)
Pngase f, by New England Biolabs (2 mentions)
Anti foxo3, by Merck Group (1 mentions)
2 protocols using anti man1a1 m
1
Antibody Sources and Inhibitors for O-GlcNAcylation Analysis
2
Antibodies and Inhibitors for O-GlcNAc Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies were obtained from several sources: anti-O-GlcNAc (RL-2, #MA1-072) from Pierce Biotechnology (Rockford, IL); anti-MAN1A1 (#M-3694) from Sigma-Aldrich (St. Louis, MO); anti-pAkt (ser-473, #9271s), anti-Akt, anti-pFOXO3 (ser-294, #5538s), and anti-pFOXO3 (thr-32, #9464s) from Cell Signaling (Danvers, MA); anti-Erk (K-23, #sc-94), anti-pErk (E-4, #sc-7383), anti-pIkk (T23, #sc-101706), and anti-Ikk (H470, #sc-7607) from Santa Cruz Biotechnology; anti-FOXO3 (#07–702) and anti-GAPDH (#MAB-374) from Merck Millipore (Billerica, MA). Akt inhibitor, MK-2206 dihydrochloride (#sc-364537) was from Santa Cruz Biotechnology (Santa Cruz, CA) and Erk inhibitor, PD98059 (#9900) was obtained from Cell Signaling (Danvers, MA). PNGase-F (#P0704) was purchased from New England Biolabs (Ipswich, MA), Pisum Sativum Agglutinin (PSA, #L-1050), biotinylated PSA (#B-1055), biotinylated Concanavalin A (#B-1005) were obtained from Vector Laboratories (Burlingame, CA). Mannosidase I inhibitor, kifunensine (#K1140), OGA inhibitor, [O-(2-Acetamido-2-deoxy-D-glucopyranosylidenamino)-N-phenylcarbamate; PUGNAc, #A7229], and cycloheximide (CHX, #C104450) were purchased from Sigma-Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!