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Ap 1 luciferase reporter plasmid

Manufactured by Addgene
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The AP-1 luciferase reporter plasmid is a molecular biology tool used to measure the activity of the AP-1 transcription factor. It consists of a luciferase reporter gene under the control of a promoter element that responds to AP-1 activation. This plasmid can be used to monitor changes in AP-1 signaling in cells.

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Lab products found in correlation

P3 primary cell nucleofector kit, by Lonza (2 mentions) Soluble anti cd28 cd28.2, by BD (2 mentions) Human il 2, by Thermo Fisher Scientific (2 mentions) Prl sv40 renilla luciferase reporter, by Promega (2 mentions) Anti cd3 cd3 2, by Mabtech (2 mentions) Dual luciferase reporter assay system, by Promega (2 mentions) Amaxa nucleofector system, by Lonza (2 mentions)

2 protocols using ap 1 luciferase reporter plasmid

1

T Cell Activation and Transcriptional Assay

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Naive CD4+ T cells were activated with anti-CD3/anti-CD28 beads and transduced with a lentiviral vector as described above. After 36 hours, activated cells were cotransfected with the AP-1 luciferase reporter plasmid (Plasmid #40342; Addgene) along with pRL-SV40 renilla luciferase reporter (Promega) using the Amaxa Nucleofector system and P3 primary cell Nucleofector Kit (Lonza). After 4 hours, activated cells were cultured on plates coated with 1 μg/ml anti-CD3 (CD3–2; Mabtech) plus 2 mg/ml soluble anti-CD28 (CD28.2; BD Biosciences) and 10 U/ml human IL-2 (Peprotech). On day 3 after activation, cells were lysed and luciferase reporter activity was measured with the Dual-Luciferase Reporter Assay System (Promega).
Kim C., Hu B., Jadhav R.R., Jin J., Zhang H., Cavanagh M.M., Akondy R.S., Ahmed R., Weyand C.M, & Goronzy J.J. (2018). Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells. Cell reports, 25(8), 2148-2162.e5.
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2

T Cell Activation and Transcriptional Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naive CD4+ T cells were activated with anti-CD3/anti-CD28 beads and transduced with a lentiviral vector as described above. After 36 hours, activated cells were cotransfected with the AP-1 luciferase reporter plasmid (Plasmid #40342; Addgene) along with pRL-SV40 renilla luciferase reporter (Promega) using the Amaxa Nucleofector system and P3 primary cell Nucleofector Kit (Lonza). After 4 hours, activated cells were cultured on plates coated with 1 μg/ml anti-CD3 (CD3–2; Mabtech) plus 2 mg/ml soluble anti-CD28 (CD28.2; BD Biosciences) and 10 U/ml human IL-2 (Peprotech). On day 3 after activation, cells were lysed and luciferase reporter activity was measured with the Dual-Luciferase Reporter Assay System (Promega).
Kim C., Hu B., Jadhav R.R., Jin J., Zhang H., Cavanagh M.M., Akondy R.S., Ahmed R., Weyand C.M, & Goronzy J.J. (2018). Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells. Cell reports, 25(8), 2148-2162.e5.
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