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3 protocols using chlorin e6 ce6

1

Photodynamic Therapy Nanoparticle Synthesis

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Poly(ethylene glycol) methyl ether acrylate (PEGA), Ethyl 2-bromoisobutyrate (EBIB), and Tris [2-(dimethylamino) ethyl] amine were purchased from Sigma-Aldrich (Shanghai, China). n-Butyl acrylate and Isopropyl alcohol (IPA), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Chlorin e6(Ce6), and Perfluoro-15-crown-5-ether (PFCE) were obtained from Macklin (Shanghai, China). Fetal bovine serum (FBS) was purchased from Every Green (Hangzhou, China). Dulbecco’s Modified Eagle Medium (DMEM), Phosphate-Buffered Saline (PBS), and Trypsin were purchased from Solarbio (Beijing, China). 2′,7′-Dichlorodihydrofluorescein diacetate, Singlet Oxygen Sensor Green, and Hoechst 33342 were obtained from Beyotime (Shanghai, China). All of the chemicals were used as supplied without further purification.
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2

Ferroptosis-Inducing Photodynamic Therapy

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The PZT was obtained from SCH Technology Co. Ltd. The control circuit with 660 nm µLEDs was obtained from Inper LLC. The PDMS Sylgard184 was purchased by Dow Corning. Lung cancer cell line LLC, A549, and H1299 were obtained from the Cell Bank of Chinese Academy of Sciences and cultured in Dulbecco's modified Eagle's medium (Yeasen, China) supplemented with 10% (v/v) fetal bovine serum (40 130, Yeasen, China). Chlorin e6 (Ce6) was obtained from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Imidazole ketone erastin (IKE) was purchased from Selleck Chemicals Co., Ltd. (Shanghai, China). Female BALB/c mice aged six to eight‐week were purchased from Beijing HFK Bioscience Co., Ltd. (www.hfkbio.com). Anti‐xCT (ab307601), GPX4 (ab125066), and 4‐HNE (ab46545) primary antibodies were purchased from Abcam (Shanghai, China). Anti‐FTH1 (A19544), TfR1 (A5865), ACSL4 (A6826), 4‐HNE (A24456), and β‐actin (AC026) primary antibodies were purchased from Abclonal (Wuhan, China). D‐Luciferin, sodium salt (40 902) was purchased from Yeasen (Shanghai, China).
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3

Synthesis and Characterization of GlcFe-Ce6 Conjugate

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Ferrous gluconate hydrate (GlcFe) and chlorin e6 (Ce6) were purchased from Macklin Reagent Inc. (Shanghai, China). N,N′-Dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), and dimethyl sulfoxide (DMSO) were purchased from Aladdin Industries Inc. (Shanghai, China). All the reagents were used directly without further purification. Deionized (DI) water was used throughout the whole experiments.
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