Log In Sign up
The largest database of trusted experimental protocols

S1pr1

Manufactured by R&D Systems
Visit Supplier

S1PR1 is a transmembrane G protein-coupled receptor that binds the lysophospholipid sphingosine 1-phosphate (S1P). S1PR1 regulates cell migration and differentiation, and plays a role in immune system function.

Automatically generated - may contain errors

Lab products found in correlation

Fixable viability dye, by Thermo Fisher Scientific (2 mentions) Cd64 x54 5 7, by BioLegend (1 mentions) Cd44 im7, by Thermo Fisher Scientific (1 mentions) Cd8a 53 6, by Thermo Fisher Scientific (1 mentions) Mhcii m5 114.15.2, by Thermo Fisher Scientific (1 mentions) Cd69 h1.2f3, by BD (1 mentions) Cd45.1 a20, by Thermo Fisher Scientific (1 mentions) Cd4 rm4 5, by Thermo Fisher Scientific (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions) Foxp3 fix perm buffer set, by BioLegend (1 mentions) Facsaria fusion cell sorter, by BD (1 mentions) S1pr1, by Merck Group (1 mentions) Anti biotin magnetic beads, by Miltenyi Biotec (1 mentions) Fluorochrome conjugated antibodies, by BioLegend (1 mentions) Dnase 1, by Merck Group (1 mentions) Facscanto 2, by BD (1 mentions) Dispase, by Roche (1 mentions) Collagenase p, by Roche (1 mentions) Alexa 488 anti rabbit, by Jackson ImmunoResearch (1 mentions) Nogo b, by R&D Systems (1 mentions)

4 protocols using s1pr1

1

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were incubated with a fixable viability dye (eBioscience) for 20 min at 4°C. Samples were blocked with FC block (24G2 grown in house and mouse serum) for 20 min followed by antibody staining for 20 min. Antibodies used: CD45.1 (A20, eBioscience), CD4 (RM4-5, eBioscience), Va2 (B20.1, BD), MHC II (M5/114.15.2, eBioscience), CD64 (X54-5/7.1, BioLegend), CD8a (53-6.7, eBioscience), CD103 (M290, BD Horizon), Ly6G (1A8 BD), CD69 (H1.2F3, BD), S1PR1 (713412, R&D Systems), interferon-γ (IFN-γ) (XMG1.2, BioLegend), and CD44 (IM7, eBioscience). Samples were washed twice with FACS buffer and acquired on a Miltenyi Macsquant analyzer. Samples were analyzed using FlowJo (Treestar) version 9.7.5.
Jaigirdar S.A., Benson R.A., Elmesmari A., Kurowska-Stolarska M.S., McInnes I.B., Garside P, & MacLeod M.K. (2017). Sphingosine-1-Phosphate Promotes the Persistence of Activated CD4 T Cells in Inflamed Sites. Frontiers in Immunology, 8, 1627.
+ Open protocol
+ Expand
2

Isolation and Analysis of Lymph Node Cells

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
dLNs (2 inguinal, 2 axillary, 2 brachial, 2 popliteal, and 4 cervical LNs per mouse) were isolated and digested as described (45 (link)) in RPMI 1640 with 2% FBS, 0.8 mg/ml Dispase (Roche Diagnostics), 0.2 mg/ml Collagenase P (Roche Diagnostics), and 0.1 mg/ml DNase I (Sigma-Aldrich). Single cell suspensions were stained in the presence of Fc-block with fluorochrome-conjugated antibodies (all purchased from BioLegend unless otherwise indicated) against the following targets: CD4 (RM4-4), CD45 (30-F11), CD3 (1452C11), TCRb (H57-597), CD44 (IM7), CD62L (MEL-14), CD69 (H1.2F3), gp38 (8.1.1), CD31 (MEK13.3), S1PR1 (713412, R&D Systems). Staining with S1PR1 antibody was performed in the presence of fatty acid–free BSA from Sigma-Aldrich; blood cells were used as S1PR negative controls (17 (link), 46 (link)). Stained cells were analyzed with the FACS Canto II (BD Biosciences) and Flow-Jo software. For ki67 (clone 16A8, BioLegend) intracellular staining, cells were fixed and permeabilized with the Foxp3 Fix/Perm Buffer Set (BioLegend). LN stromal cells were sorted as described (45 (link)) using a BD FACSAria Fusion cell sorter; CD45+ cells were depleted prior to sorting with anti-biotin magnetic beads (Miltenyi Biotec).
Urso K., Alvarez D., Cremasco V., Tsang K., Grauel A., Lafyatis R., von Andrian U.H., Ermann J, & Aliprantis A.O. (2016). IL4RA on lymphatic endothelial cells promotes T cell egress during sclerodermatous graft versus host disease. JCI Insight, 1(12), e88057.
+ Open protocol
+ Expand
3

Immunofluorescence Analysis of Placental Arteries

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated arterial chorionic placental vessels were incubated in calcium-free Krebs for at least 30 min to allow vessel vasodilation. Subsequently, the arteries were fixed with 4% PFA and left overnight at 4 °C. PFA-fixed arteries were OCT-embedded. For immunofluorescence, frozen placental artery sections were stained for Nogo-B (1:200, R&D), S1PR1 (1:200, R&D), and CD31 (1:200, Invitrogen) overnight at 4 °C and were then stained with Cy5-labeled anti-goat antibody (#A21436, Invitrogen, 1:500) Alexa 488 anti-rabbit (#016-540-084, Jackson ImmunoResearch, 1:200) and Alexa 568 anti-mouse in PBS for 1 h. Nuclei were stained with DAPI. Confocal immunofluorescence images of the tissues were captured on an Olympus Fluoview confocal microscope and quantified with ImageJ.
Del Gaudio I., Sasset L., Di Lorenzo A, & Wadsack C. (2020). Sphingolipid Signature of Human Feto-Placental Vasculature in Preeclampsia. International Journal of Molecular Sciences, 21(3), 1019.
+ Open protocol
+ Expand
4

Comprehensive Immune Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with fluorochrome-labeled antibodies to perforin, granzyme B, CXCR3, Bcl6, Eomes, fixable viability dye (eBiosciences, San Diego, CA), CD44, CD62L, Ly6C, IL-10 (Biolegend, San Diego, CA), Tcf1 (Cell Signaling Technology, Danvers, MA), S1PR1 (R&D, Minneapolis, MN), IFNυ, TCRαβ and CD8 (BD Biosciences, San Jose, CA). Flow data were acquired on an Accuri C6 or LSRII (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star Inc., Ashland, OR).
Reiser J., Sadashivaiah K., Furusawa A., Banerjee A, & Singh N. (2018). Eomesodermin driven IL-10 production in effector CD8+ T cells promotes a memory phenotype.. Cellular immunology, 335, 93-102.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!