Cardiomyocytes were cultured onto a 24-well plate with glass cover slips at a density of 5 × 104 cells/well. The cells were then fixed with 4% paraformaldehyde for 20 min and quenched with 1 M ethanolamine diluted in PBSc. After washing, cells were blocked with 0.5% bovine serum albumin in PBS for 30 min, then the blocking solution was removed and the cells were incubated overnight at 4 °C with rabbit anti-AhR (1:100 dilutions, BioWorld). Cells were washed and incubated with mouse anti-rabbit IgG-TR (1:1000 dilutions, Santa Cruz Biotechnology) at room temperature for 1 h. Then, the cells were gently washed under the cover slip three times with PBS and visualized under a laser scanning confocal microscope (Fluoview FV1000 confocal system, Olympus).
Mouse anti rabbit igg tr
Manufactured by Santa Cruz Biotechnology
Mouse anti-rabbit IgG-TR is a secondary antibody conjugated with Texas Red (TR) fluorescent dye. It is designed to detect and visualize rabbit primary antibodies in immunoassays and other applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse anti rabbit igg tr
1
Immunofluorescence Staining of AhR in Cardiomyocytes
2
Immunofluorescence Staining of ER-alpha and NF-κB
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Cells cultured on cover slides were fixed with 2% paraformaldehyde for 20 minutes, followed by three washes with PBS with Tween (PBST). The cells were then blocked using PBS containing 10% serum and Triton X-100 for 1 hour at room temperature. Subsequently, the cells were incubated with rabbit anti-ER alpha antibody and NF-κB overnight at 4 °C. After washing three times with PBST, the cells were incubated for 45 minutes at room temperature with secondary antibodies that were diluted in the blocking buffer. These secondary antibodies included FITC-mouse anti-rabbit immunoglobulin G (IgG) and mouse anti-rabbit IgG-TR (Santa Cruz Biotechnology). The cells
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