Phospho met clone d26

Manufactured by Cell Signaling Technology

Phospho-MET clone D26 is a rabbit monoclonal antibody that recognizes the tyrosine phosphorylated form of the MET receptor. It is intended for use in immunochemical techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect the activated, phosphorylated form of the MET protein.

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Lab products found in correlation

Met clone d1c2, by Cell Signaling Technology (1 mentions) Phospho akt clone d9e, by Cell Signaling Technology (1 mentions) Phospho erk clone d13.14.4e, by Cell Signaling Technology (1 mentions)

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Phospho-Met (24 citations)

2 protocols using phospho met clone d26

Tissue Microarray Staining for MET

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Tissue microarrays were previously described (20 ). All specimens were collected through an institutionally-approved protocol with the consent of the patient and in accordance with federal ethical guidelines. Five micron sections were stained using the SP44 clone antibody on a Ventana Discovery instrument according to manufacturer’s instructions. For patient-derived xenografts, staining was performed with MET clone D1C2 (Cell Signaling) and phospho-MET clone D26 (Cell Signaling).

Smith M.A., Licata T., Lakhani A., Garcia M.V., Schildhaus H.U., Vuaroqueaux V., Halmos B., Borczuk A.C., Chen Y.A., Creelan B.C., Boyle T.A, & Haura E.B. (2017). MET-GRB2 Signaling-Associated Complexes Correlate with Oncogenic MET Signaling and Sensitivity to MET Kinase Inhibitors. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(22), 7084-7096.

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Western Blot Analysis of Signaling Proteins

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Western blotting was performed as previously described (25 (link)). Primary antibodies for phospho-ERK (clone D13.14.4E; catalog #4370S), total ERK (clone 137F5; catalog #4695S), phospho-AKT (clone D9E; catalog #4060S), total AKT (clone 40D4; catalog #2920S), phospho-Met (clone D26; catalog #3077S), and total Met (clone D1C2; catalog #8198S) from Cell Signaling Technology.

Phadke M.S., Chen Z., Li J., Mohamed E., Davies M.A., Smalley I., Duckett D.R., Palve V., Czerniecki B.J., Forsyth P.A., Noyes D., Adeegbe D.O., Eroglu Z., Nguyen K.T., Tsai K.Y., Rix U., Burd C.E., Chen Y.A., Rodriguez P.C, & Smalley K.S. (2021). Targeted therapy given after anti-PD-1 leads to prolonged responses in mouse melanoma models through sustained antitumor immunity. Cancer immunology research, 9(5), 554-567.

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