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Leica led5000 hdi

Manufactured by Leica camera
Sourced in Germany

The Leica LED5000 HDI is a high-performance LED illumination system designed for microscopy applications. It provides stable, uniform illumination with adjustable intensity to enhance observation and imaging quality.

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3 protocols using leica led5000 hdi

1

Genitalia Morphology of South Korean Insects

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Specimens examined are preserved in the collections of the Incheon National University, South Korea (INU) and the Korean National Arboretum, Pocheon, Korea (KNAE, HEO). Genitalia were dissected and examined using a Leica EZ4 stereomicroscope. Images of adults were taken by a Tucsen Dhyana 400DC digital camera attached to a Leica S6D stereomicroscope, with dome illuminator Leica LED5000 HDI. Genitalia photograph were taken using a Tucsen Dhyana 400DC digital camera mounted on a Leica S8AP0 stereomicroscope.
Further abbreviations:

CB: Chungcheongbuk-do Province.

GG: Gyeonggi-do Province.

GW: Gangwon-do Province.

HEO: Un-Hong Heo’s private collection.

JB: Jeollabuk-do Province.

JJ: Jeju-do Island.

JN: Jeollanam-do Province.

NHMUK: The Natural History Museum, London, United Kingdom

NIAES: National Institute for Agro-Enviromental Sciences, Ibaraki, Japan.

ZI: Zoological Institute, Academy of Sciences of the U.S.S.R., Saint Petersburg (Leningrad), Russia.

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2

High-quality Insect Specimen Photography

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The photographs (Figure 1) were taken following the method described by Taszakowski and Kaszyca [54 (link)]. To prepare high-quality photos that would enable advanced processing (e.g., obtaining a uniform background), the specimens were stuck onto transparent entomological glue boards and then cleaned with a thin brush. To improve the visibility of details, pictures of the labia were taken with the use of a dark background. The focus-stacked, colour photographs were captured using the following equipment: Leica M205C (stereomicroscope), Leica LED5000 HDI (high diffuse dome illumination), Leica DFC495 (digital camera), and Leica application suite 4.12.0 (software) (Leica Microsystems, Vienna, Austria). The resulting photographs were composed and enhanced with Image Composite Editor (panoramic image stitcher) and Adobe Photoshop CS6 graphic editor.
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3

Comparative Morphology of Heterodasypoda Bees

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Based on the results of the genetic barcoding (see below), we further examined the morphology of these females in comparison with that of the males and that of the other species of Heterodasypoda (i.e. Dasypoda albimana, D. morotei and D. pyrotrichia). High-quality pictures of both sexes of D. michezi were taken using a Canon EOS 5DS R (Canon Inc., Tokyo, Japan) camera assembled onto a stereomicroscope Leica M205C (Leica Microsystems, Wetzlar, Germany) with Leica LED5000 HDI illuminator under Helicon Remote 3.9.7.w. software. Photographs were then combined into a single image using Helicon Focus 7.5.6 Pro (Helicon Soft Ltd, Kharkiv, Ukraine) automontage software.
Finally, we established a key to all known species of Heterodasypoda following Michez et al. (2004a, b) and the results of the present study. The following abbreviations, after Michener (2007) , are used below: T1, T2… = first, second, etc. metasomal terga; S1, S2… = first, second, etc. metasomal sterna; A1, A2… = first, second, etc. antennal segments, pw = puncture width.
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