Ion pitm template ot2 200 kit v3

Sample emulsion PCR, emulsion breaking, and enrichment were performed using the Ion PI^TM Template OT2 200 Kit v3 (Life Technologies, Part #4488318 Rev. B.0) according to the manufacturer's instructions. Equal molar ratios of multiple barcoded libraries were combined for one Ion PI^TM v2 chip. Two pooled Ion AmpliSeq^TMExome libraries were loaded onto a single Ion PI^TM v2 chip. Five pooled Ion AmpliSeq™ Transcriptome libraries were loaded onto a single Ion PI^TM v2 chip. Subsequent emulsion PCR and enrichment of the pooled library sequencing beads was performed using the Ion OneTouch^TM system (Life Technologies) according to the manufacturer's protocol within approximately seven hours. Finally, sequencing (520 flows) was performed with the Ion PI^TM v2 chip using the Ion PI^TM Sequencing 200 Kit v3 (Life Technologies, Part #4488315 Rev. B.0) on the Ion Proton^TM sequencer (Life Technologies).

Sample emulsion PCR, emulsion breaking, and enrichment were performed using an Ion PITM Template OT2 200 Kit v3 (Life Technologies, Part #4488318 Rev. B.0) according to the manufacturer’s instructions. Multiple barcoded libraries were combined with equal molar ratios for one Ion PITM v2 chip. Two‐pooled Ion AmpliSeqTM Exome libraries were loaded onto a single Ion PITM v2 chip. Five‐pooled Ion AmpliSeq™ Transcriptome libraries were loaded onto a single Ion PITM v2 chip. Subsequent emulsion PCR and enrichment of the sequencing beads of the pooled libraries were performed using the Ion OneTouchTM system (Life Technologies) within approximately 7 h, according to the manufacturer’s protocol. Finally, 520 Flows sequencing was done on the Ion PITM v2 chip using Ion PITM Sequencing 200 Kit v3 (Life Technologies, Part #4488315 Rev. B.0) on the Ion ProtonTM sequencer (Life Technologies).