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Positive cytoplasm control probe 18s

Manufactured by RiboBio
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The Positive cytoplasm control probe 18S is a laboratory reagent designed to detect the presence of 18S ribosomal RNA, a component of the small subunit of the eukaryotic ribosome. This probe serves as a positive control to validate the functionality of RNA detection procedures.

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Lab products found in correlation

Positive nucleus control probe u6, by RiboBio (2 mentions) Fish kit, by RiboBio (1 mentions) Tcs sp8, by Leica (1 mentions) Fluorescent in situ hybridization kit, by RiboBio (1 mentions)

Close spelling variants of this product

18S probes Control probes

2 protocols using positive cytoplasm control probe 18s

1

Quantitative LINC00460 lncRNA Detection

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RNA FISH assay was performed with the Ribo FISH Kit (Ribo, China) according to the manufacturer's instructions. The lncRNA probe mix of LINC00460 and positive nucleus control probe U6 and positive cytoplasm control probe 18S were designed and purchased from Ribo. Images of cultured cells were obtained using the ZEISS Laser Scanning Confocal Microscope LSM710 with oil ×67 magnification lenses, and ZEN 2.3 software was used.
Zhu X., Li J., Ni X., Wu X., Hou X., Li Y., Li S., Zhao W, & Yin X. (2022). Pancreatic ductal adenocarcinoma cells regulated the gemcitabine‐resistance function of CAFs by LINC00460. Cancer Science, 113(11), 3735-3750.
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2

lncRNA Expression Profiling by RNA FISH

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RNA FISH assay was performed with Ribo Fluorescent in Situ Hybridization Kit (Ribo, China) according to manufacturer’s instructions. The lncRNA probe mix of u50535 and positive nucleus control probe U6 and positive cytoplasm control probe 18S were designed and purchased from Ribo company. The FISH results were captured by confocal instrument (Leica TCS-SP8).
Yu X., Yuan Z., Yang Z., Chen D., Kim T., Cui Y., Luo Q., Liu Z., Yang Z., Fan X., Chen D, & Wang L. (2018). The novel long noncoding RNA u50535 promotes colorectal cancer growth and metastasis by regulating CCL20. Cell Death & Disease, 9(7), 751.
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