Anti human cd163
Anti-human CD163 is a monoclonal antibody that specifically binds to the CD163 protein, a cell surface receptor expressed primarily on monocytes and macrophages. CD163 plays a role in the clearance of hemoglobin-haptoglobin complexes.
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5 protocols using anti human cd163
Macrophage Phenotyping Protocol
Sorting CD163+CD14+ and CD163-CD14+ Cells
Multimodal Immunophenotyping of Tumor Samples
For staining of cell sliders, mouse primary cells were seeded in well-plates with glass slides placed in advance. Exfoliated cells from patients or sorted mouse cells from FACS were spotted in slides by Thermo Scientific Cytospin 4 Cytocentrifuge. The immunofluorescence staining procedures were consistent with tumor tissue sections above. The Giemsa staining was performed by commercial assay (BASO BA4007), imaged by Leica DM2500 microscope.
For TUNEL assays, the formalin treated mouse tumor tissues were sectioned by paraffin embedding. And the staining procedures followed manufacturer's protocol (Promega G7130), with images obtained by Leica DM2500 microscope.
Macrophage Polarization Profiling by Flow Cytometry
Exploring Immune Profiles in Active and Inactive AOSD
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