First, 5.70 g of Zn(NO3)2·6H2O (Aladdin, AR) and 4.73 g of 2-methylimidazole (Aladdin, AR) were dissolved in 45 mL methanol (Sinopharm, AR) one after another to make a high concentration of the reactant solution. Then, around 4 g of washed polystyrene template was soaked in the above-mentioned reactant solution for 1 h and then placed under a negative pressure of −0.8 MPa for 10 min to guarantee as much reactant immersing into the void of polystyrene templates as possible. The treated templates were taken out and dried at 60 °C overnight (denoted as preZIF-8@Fcc-PS). Furthermore, preZIF-8@Fcc-PS were soaked in 60 mL of methanol and ammonia (Sinopharm, AR, 25–28%) mixed solvent (1 : 1 ratio, by volume) under −0.6 MPa for 3 min before the mixture stood at normal temperature and pressure for 24 h. At this stage, preZIF-8@Fcc-PS would split into pieces resulting from the crystallization force. Subsequently, the mixed solvent was poured away and the obtained fragments were dried for several hours (ZIF-8@Fcc-PS). At last, ZIF-8@Fcc-PS was immersed into dimethyl formamide (DMF, Sinopharm, AR) for 24 h to remove the polystyrene sphere templates, and this process was repeated 5 times in order to wash completely.
Methanol
Manufactured by Sinopharm
Sourced in China, United States
Methanol is a chemical compound with the formula CH3OH. It is a colorless, volatile, and flammable liquid used as a solvent, fuel, and chemical feedstock. Methanol has various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Sinopharm (138 mentions)
Sodium hydroxide (naoh), by Sinopharm (72 mentions)
Hydrochloric acid (hcl), by Sinopharm (70 mentions)
Acetone, by Sinopharm (69 mentions)
Acetonitrile, by Sinopharm (60 mentions)
Chloroform, by Sinopharm (49 mentions)
Toluene, by Sinopharm (43 mentions)
Sodium chloride (nacl), by Sinopharm (38 mentions)
Ethyl acetate, by Sinopharm (37 mentions)
Dichloromethane, by Sinopharm (34 mentions)
Acetic acid, by Sinopharm (29 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (29 mentions)
Formic acid, by Sinopharm (25 mentions)
N hexane, by Sinopharm (25 mentions)
N n dimethylformamide (dmf), by Sinopharm (25 mentions)
Isopropanol, by Sinopharm (22 mentions)
Hexane, by Sinopharm (21 mentions)
Cyclohexane, by Sinopharm (21 mentions)
Anhydrous ethanol, by Sinopharm (21 mentions)
Sulfuric acid, by Sinopharm (17 mentions)
520 protocols using methanol
1
Synthesis of ZIF-8 in Polystyrene Template
2
Synthesis of Metal-Organic Frameworks
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Iron(iii ) nitrate nonahydrate (Fe(NO3)3·9H2O, AR), phosphomolybdic acid (H3PMo12O40, PMA, AR), 1,3,5-benzene tricarboxylic acid (H3-BTC) (AR), absolute ethanol (AR), oleic acid (AR), methanol (AR, >99%), lauric acid (AR, 98%), stearic acid (AR, 98%), myristic acid (AR, 98%), palmitic acid (AR, 98%) and methanol (AR, >99%), were purchased from Sinopharm Chemical Regent Co., Ltd. All other chemicals were of analytical grade and used as received, unless otherwise noted.
3
Silica Aerogel Synthesis Protocol
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Methyltrimethoxysilane (MTMS, Aladdin, Shanghai, China, 98%), hydrochloric acid (HCl, Aladdin, Shanghai, China), hexadecyltrimethylammonium chloride (CTAC, Aladdin, Shanghai, China, 97%), methanol (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China, ≥99.5%), propylene oxide (PO, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China, ≥99.5%), methanol (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China, ≥99.7%), 2-propanol (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China, ≥99.7%), n-heptane (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China, ≥98.5%) were used as obtained.
4
Metabolomic Analysis of Traditional Chinese Herbs
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Analytical grade methoxyamine hydrochloride, methanol, ethanol, chloroform, and pyridine were obtained from China National Pharmaceutical Group Corporation in Shanghai of China. N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA:TMCS, 99 : 1), heptadecanoic acid and urease were purchased from Sigma-Aldrich. Liquor (56 %, v/v, 00215) was obtained from Beijing Red Star, China. Double-distilled water was produced by a Milli-Q Ultra-pure water system (Millipore Corporation, United States). Dry S. baicalensis and B. chinense were purchased from Inner Mongolia and An’hui was identified as S. baicalensis Georgi and B. chinense DC by Dr. Lihong Wu of Shanghai University of Traditional Chinese Medicine. Baicalin and Saikosaponin were purchased from Ciyuan Biotechnology (Shanxi, China).
5
Gelatin Extraction and Characterization
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Type A gelatin from pigskin was purchased from China National Pharmaceutical Group Corporation (Beijing, China) and used after dialysis. The isoelectric point (pI, 8.5) of the dialyzed gelatin after complete deionization was determined by fluorescence spectroscopy. Sodium dodecyl sulfate (SDS) was purchased from Alfa Aesar and recrystallized from ethanol before use. Ally glycidyl ether (AGE) and chloroplatinic acid hexahydrate (H2Pt6Cl6•6H2O) were obtained from Alfa Aesar. Hexamethylcyclotrisiloxane (D3, >95%), n–butyllithium (C4H9Li, >99%), and chlorodimethylsilane (C2H7ClSi, >99%) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Benzene, deionized water (conductivity = 2.06 µS cm−1, dielectric constant ε = 80.40), methanol (ε = 32.70), ethanol (ε = 24.50), isopropanol alcohol (ε = 17.90), acetone (ε = 20.70), tetrahydrofuran (THF, ε = 7.58), and 1,4-dioxane (ε = 2.25) solvents (China National Pharmaceutical Group Corporation) were all of analytical reagent (AR) grade and strictly dehydrated before use.
6
Quantitative Metabolic Profiling Assay
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Methanol and 2-chlorophenylalanine were of analytical grade from China National Pharmaceutical Group Corporation (Shanghai, China). Heptadecanoic acid, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and methoxyamine hydrochloride were obtained from Sigma-Aldrich (St. Louis, MO, USA). Commercial kits used for determining alanine aminotransferase (ALT), aspartate aminotransferase (AST), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG) were obtained from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China).
7
Citrus Bioactive Compound Analysis
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Fresh citrus reticulata fruits were provided by Shanghai Manqin fruit industry Co., Ltd. (Shanghai, China). In addition, 1-, 5- and 10-year storage CRP (Xinhui CRP) were purchased by Jiangmen GanZhiXiang Food Co., LTD.
Hesperidin (≥98%) and nobiletin (≥98%) were purchased from Shanghai YuanYe Bio-Technology Co., Ltd., and hesperetin (≥98%) was purchased from Shanghai NatureStandard Technical Services Co., LTD. Acetonitrile and methanol were purchased from China National Pharmaceutical Group Co., Ltd. with HPLC-grade. All other solvents were of analytical grade.
Hesperidin (≥98%) and nobiletin (≥98%) were purchased from Shanghai YuanYe Bio-Technology Co., Ltd., and hesperetin (≥98%) was purchased from Shanghai NatureStandard Technical Services Co., LTD. Acetonitrile and methanol were purchased from China National Pharmaceutical Group Co., Ltd. with HPLC-grade. All other solvents were of analytical grade.
8
Quantitative Detection of CRP and PCT
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Goat anti-mouse IgG, mouse monoclonal CRP antibody (CRP135 mAb), mouse monoclonal PCT antibody (14C12 mAb), its paired mouse monoclonal calcitonin antibody (14A2 mAb), CRP-free serum, and PCT-free serum were obtained from Hytest. Analytically pure reagents: cadmium oxide (CdO), selenium powder (Se), powdered sulfur (S), oleic acid (OA), zinc oxide powder (ZnO), octadecene (ODE), octadecyl amine (ODA), trioctylphosphine oxide (TOPO), sodium azide were obtained from Sigma-Aldrich; mercaptoacetic acid (MAA), trichloromethane, normal hexane, methanol, acetone, heptane, glycine, sodium tetraborate, and sucrose were from China National Pharmaceutical Group. Standard human CRP and standard human PCT, bovine serum albumin (BSA), and phosphate-buffered saline (PBS) were from Sigma-Aldrich. Guarantee reagents: N-hydroxysuccinimide (NHS) and carbodiimide (EDC) were from China National Pharmaceutical Group. Nitrocellulose membrane and glass cellulose membrane were from Millipore; the whole blood separator was from Shanghai JieYi Biotechnology; and the stickiness baseplate was from Shanghai Liangxin Science and Technology Ltd. Ultrapure water (Milli-Q) is used in the detections.
9
Synthesis of a Zr-based MOF
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Zirconium tetrachloride (ZrCl4), N,N-dimethyl formamide (DMF), glacial acetic acid (HAc), and methanol were obtained from Sinopharm Chemical Reagent Co., Ltd. Na2WO4·2H2O and Bi(NO3)3·5H2O were purchased from Aladdin Industrial Corporation. 2-NH2-terephthalic acid was procured from Sigma-Aldrich Chemistry. All the reagents were of analytical grade and were used without further purification.
10
Fungal Fermentation of Jujube Fruit
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The materials and reagents required for this experiment were as follows: jujubes were purchased from the market. A. niger (H1) was isolated from the wine-producing region in the Hexi Corridor, Gansu Province, and was provided by the College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China. Agar was purchased from Beijing Kulaibo Technology Co., Ltd., Beijing, China. YES medium was obtained from Thermo Fisher Scientific, Shanghai, China. Methanol was purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Acetonitrile was purchased from Chengdu Kelon Chemical Co., Ltd., Chendu, China. Ascorbic acid was obtained from Tianjin New Fine Chemical Development Center, Tianjin, China. Phenolphthalein was acquired from Shanghai Zhongqin Chemical Reagent Co., Ltd., Shanghai, China. The supplier of 3,5-Dinitrosalicylic acid was purchased from Zhengzhou Huiju Chemical Co., Ltd., Zhenzhou, China. Folin-phenol reagent was purchased from Tianjin Guangfu Technological Development Co., Ltd., Tianjin, China.
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