Got ast lq

Lipase, amylase and aspartate aminotransferase (AST) activity were determined in plasma by the LIPASE-LQ, AMYLASE-LQ and GOT (AST)-LQ, respectively, (Spinreact, Girona, Spain). The procedures were performed according to the indications of the kit.
Triglycerides and total lipids were determined in liver tissue by the TRIGLYCERIDES-LQ and TOTAL LIPIDS, respectively (Spinreact, Girona, Spain). The procedures were performed according to the indications of the kit in liver homogenate in PBS (100 mg/mL). Results were normalized by protein concentration.

Serum glucose (Glucose-LQ GOD-POD, Spinreact, Girone, Spain), triglycerides (Triglycerides-LQ GPO-POD, Spinreact), aspartate transaminase (GOT-AST-LQ, Spinreact), and alanine transaminase (GPT-ALT-LQ, Spinreact) were determined using commercial enzymatic-colorimetric kits following the manufacturer’s instructions. Following the manufacturers’ instructions, serum insulin was determined using an ELISA kit (EZRMI-13K, EMD Millipore Co., Burlington, MA, USA). Insulin resistance- and beta-Homeostatic model assessment (HOMA-IR and HOMA-beta), quantitative insulin sensitivity check index (QUICKI), fasting glucose-to-insulin ratio (FGIR), and fasting triglycerides and glucose (TyG) indexes were calculated with the following equations: $$\mathrm{HOMA}-\mathrm{IR}=\frac{\mathrm{glucose} \left(\raisebox{1ex}{$\mathrm{mg}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{dL}$}\right.\right)\times \mathrm{insulin}\left(\raisebox{1ex}{$\mathsf{\mu }\mathrm{M}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{mL}$}\right.\right)}{2430}$$
$$\mathrm{HOMA}-\mathrm{Beta}=\frac{\mathrm{insulin} \left(\raisebox{1ex}{$\mathrm{ng}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{mL}$}\right.\right)\times 360}{\mathrm{glucose} \left(\raisebox{1ex}{$\mathrm{mg}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{dL}$}\right.\right)-63}$$
$$\mathrm{QUICKI}=\frac{1}{\log \left[\mathrm{insulin}\left(\raisebox{1ex}{$\mathsf{\mu }\mathrm{M}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{mL}$}\right.\right)\right]+\log \left[\mathrm{glucose}\left(\raisebox{1ex}{$\mathrm{mg}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{dL}$}\right.\right)\right]}$$
$$\mathrm{FGIR}=\frac{\mathrm{glucose} \left(\raisebox{1ex}{$\mathrm{mg}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{dL}$}\right.\right)}{\mathrm{insulin} \left(\raisebox{1ex}{$\mathsf{\mu }\mathrm{M}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{mL}$}\right.\right)}$$
$$\mathrm{TyG}=\frac{\ln \left[\mathrm{glucose}\left(\raisebox{1ex}{$\mathrm{mg}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{dL}$}\right.\right)\ast \mathrm{triglycerides}\left(\raisebox{1ex}{$\mathrm{mg}$}\!\left/ \!\raisebox{-1ex}{$\mathrm{dL}$}\right.\right)\right]}{2}$$