To avoid contamination, 50% ethanol was used to clean fertilized white Lohmann chicken eggs. At a temperature of 37.5 °C and a humidity of approximately 60–70%, the eggs were positioned with an upright orientation in an incubator to induce embryogenesis (Bruja 3000 digital, Siepmann, Germany and Mini Pro 147, Maino, Italy). After optimization of our protocols, as discussed in our previously published studies, the CAM was lowered by removing 4–8 mL albumin on ED 5 with a 10 mL sterile syringe and a 20-gauge safety butterfly cannula (Safety-Multifly Needle, Sarstedt, Nümbrecht, Germany). A surgical tape was used to reseal the puncture site (3M Micropore surgical tape, Saint Paul, MN, USA). Under aseptic conditions, a window was carefully opened in the eggshell on ED 6 without damaging the underlying embryonic structures to facilitate the implantation of the generated tumor cell pellets. Subsequently, Parafilm was placed over the opening (Bemis Company Inc., Neenah, WI, USA) and the eggs were positioned back in the incubator until the implantation on ED 7.
Micropore surgical tape
Manufactured by 3M
Sourced in United States, Australia
Micropore surgical tape is a medical-grade adhesive tape designed for use in healthcare settings. It is made of a thin, porous material that allows the skin to breathe while providing a secure and gentle adhesion. The tape is intended for use in various medical applications, such as securing dressings, bandages, and other medical devices to the skin.
Automatically generated - may contain errors
Lab products found in correlation
Digital vortex genie 2, by Thermo Fisher Scientific (2 mentions)
Safety multifly needle, by Sarstedt (1 mentions)
First principles software, by Northern Digital (1 mentions)
Optotrak certus, by Northern Digital (1 mentions)
Silwet l 77, by Bioworld Technology (1 mentions)
Escitalopram oxalate, by Merck Group (1 mentions)
Tegaderm film, by 3M (1 mentions)
Methyl tert butyl ether (mtbe), by Avantor (1 mentions)
Donepezil hydrochloride, by Tokyo Chemical Industry (1 mentions)
20 protocols using micropore surgical tape
1
Chicken Egg Embryogenesis and Tumor Implantation
2
Standardized Thermographic Ocular Imaging
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Based on a previously reported method for obtaining thermographic ocular images and measurements [26 (link)], a standard protocol was maintained in all cases in order to reduce the risk of bias. Thermographic imaging was performed under controlled environmental conditions, with a fixed room temperature of 25 °C, and no air drafts. Thermographic images were taken only after at least 20 min of acclimatization to room temperature.
Prior to image capture, the room and core body temperature (per os) were recorded. Thermal images were taken from the same distance of 30 cm. Video and still thermal images were taken using Therm-App Pro TH (Opgal Optronic Industries Ltd. Karmiel, Israel) camera with a 9 mm lens (384 × 288-pixel resolution).
The subjects were instructed to place their head in the slit lamp, and look straight into the camera. During imaging, subjects were requested to breath normally with their mouth and nose covered by a standard surgical face mask (Sion Biotext medical Ltd., Maastricht, The Netherlands). The images were taken consecutively over a 30 s period, capturing the thermographic data at inspirium and expirium. After completion, 20 of the subjects were also asked to repeat the imaging after sealing the superior end of the mask using micropore surgical tape (3M, Saint Paul, MN, USA). For each participant, the right eye was used for analysis.
Prior to image capture, the room and core body temperature (per os) were recorded. Thermal images were taken from the same distance of 30 cm. Video and still thermal images were taken using Therm-App Pro TH (Opgal Optronic Industries Ltd. Karmiel, Israel) camera with a 9 mm lens (384 × 288-pixel resolution).
The subjects were instructed to place their head in the slit lamp, and look straight into the camera. During imaging, subjects were requested to breath normally with their mouth and nose covered by a standard surgical face mask (Sion Biotext medical Ltd., Maastricht, The Netherlands). The images were taken consecutively over a 30 s period, capturing the thermographic data at inspirium and expirium. After completion, 20 of the subjects were also asked to repeat the imaging after sealing the superior end of the mask using micropore surgical tape (3M, Saint Paul, MN, USA). For each participant, the right eye was used for analysis.
3
Vibration-Induced Chronic Muscle Pain
4
Forehead-Mounted Accelerometer for Head Orientation Tracking
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In the proposed sensing system, an accelerometer is attached to the forehead of the subject mounted with 3M™ Micropore™ Surgical Tape in the orientation shown in Figure 4(a) , where x-axis points toward the head of the head, y-axis points to the right-hand side of the body, and z-axis points to the front of the head. With the accelerometer attached to the forehead to comply with the defined orientation, the head would rotate along the x-axis. Therefore, the rotation angle θ and the inclination angle φ can be calculated as in (3 ) for a different orientation compared with those in (2 ). It is also represented in Figure 4(b) .
where σ = 0, if Az ≥ 0; otherwise, σ = 1. λ = 1, if Ay ≥ 0; otherwise, λ = −1.
where σ = 0, if Az ≥ 0; otherwise, σ = 1. λ = 1, if Ay ≥ 0; otherwise, λ = −1.
5
Mechanical Vibration-Induced Nociceptive Threshold
6
Metallic Grid Skin Imaging Protocol
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The metallic grids were centered over the areas of interest and attached using surgical tape (Micropore Surgical Tape [3M, MN, US]) or adhesive Mastisol (Eloquest Healthcare, MI, US). Clinical and/or dermoscopic images were acquired after grid placement.
7
Barley Powdery Mildew Resistance Screening
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Barley lines were sown in 20-cm diameter pots containing UWA Bio Mix (Richgro Garden Products, Jandakot, WA) and grown under a 12-h day–night cycle at 22 °C and 18 °C, respectively, in a climate-controlled growth room (Conviron Asia Pacific Pty Ltd, VIC, Australia). Four replicates of each DH line were planted for Bgh-inoculated and non-inoculated control samples, together with cv. Baudin as a standard susceptible control. Plants were placed in a randomised complete block design for each treatment and grown until the sixth leaf was fully expanded at around 5½ weeks post-germination, as APR is growth stage dependent and is fully effective from the fifth leaf (Ge et al. 2021 (link)).
Whole plant inoculations were conducted by first marking the borders of eight-centimetre 6th leaf sections with Micropore surgical tape (3 M, Saint Paul, MN, USA). The target sections were inoculated by gently tapping infected detached barley leaves from a height of 15 cm. Thereafter, entire plants were inoculated from a height of 50 cm. Leaf sections were harvested at 48-h post-inoculation (hpi) and flash frozen in liquid nitrogen. Susceptible DH lines and cv. Baudin were inspected at 7 dpi to confirm successful infection.
Whole plant inoculations were conducted by first marking the borders of eight-centimetre 6th leaf sections with Micropore surgical tape (3 M, Saint Paul, MN, USA). The target sections were inoculated by gently tapping infected detached barley leaves from a height of 15 cm. Thereafter, entire plants were inoculated from a height of 50 cm. Leaf sections were harvested at 48-h post-inoculation (hpi) and flash frozen in liquid nitrogen. Susceptible DH lines and cv. Baudin were inspected at 7 dpi to confirm successful infection.
8
Breast Motion Analysis during Treadmill Running
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Vertical breast displacement (VBD; cm) relative to the torso was measured using an Optotrak Certus® motion capture system (200 Hz, Northern Digital, Ontario, Canada) during dynamic treadmill running to determine whether breast motion was consistent among the different strap conditions. Two infrared-emitting diodes (2 mm diameter) were placed on each nipple using double-sided toupee tape (Creative Hair Products, Melbourne, Victoria, Australia), which was placed over micropore surgical tape (3M™ Australia, NSW). A third diode was placed on the sternal notch as a reference point to characterise trunk motion in the vertical plane. Three-dimensional motion of the three markers was recorded during each running trial for six 10-s periods using First Principles software (Version 1.2.2, Northern Digital Inc., Ontario, Canada). The average VBD (minimum from maximum during dynamic treadmill running) relative to the trunk was calculated from a representative 8-s epoch (equivalent to 15 to 20 consecutive breast cycles) for each of the six 10-s data recordings per condition.
9
Arabidopsis Bacterial Infection Assay
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Bacterial infection assay was done based on a modified protocol according to the previous report67 (link). Briefly, seven-day-old Arabidopsis seedlings were chosen to do the Xcc flood-inoculation assay. Bacteria were harvested and resuspended into 40 mL of 0.02% Silwet L-77 (bioWORLD, USA), containing 10 mM MgCl2, to a final concentration at OD = 0.1. The inoculum was dispensed into the 1/2 MS plate containing Arabidopsis seedlings, and the plates were incubated for 1 min at room temperature. Then the bacteria were removed by decantation, and the plates were sealed with 3 M Micropore Surgical Tape (3 M United States). The plates were incubated at 22 °C in the long-day growth chamber (16 h light/8 h dark). Images of bacteria-infected plants were taken at the indicated time points.
10
Inducing Parasitism in Arabidopsis thaliana
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After germination, 5-day-old seedlings of C. campestris were placed in a position to attach to the inflorescence stems of 4–5-week-old A. thaliana plants. Parasitism was induced under blue light (wavelength peak = 444 nm, 7 μmol m−2 s−1) in a growth chamber at 25°C for 2 days, after which plants were grown under continuous white light (45 μmol m−2 s−1) at 22°C.
Parasitism was also induced using excised lateral shoots from mature C. campestris plants. Lateral shoots (3 cm in length) with the apex attached were cut from mature C. campestris plants that had parasitized a host plant. Shoot segments were then attached to new inflorescence stems of 4–5-week-old A. thaliana using surgical tape (Micropore™ Surgical Tape, 3M Company) and parasitism induced under blue light at 25°C as for seedlings. The process of parasitism was recorded by time-lapse imaging (TLC200, Brinno). The time at which coiling of C. campestris around the host plant was complete was designated as 0 hours after coiling (hac).
Parasitism was also induced using excised lateral shoots from mature C. campestris plants. Lateral shoots (3 cm in length) with the apex attached were cut from mature C. campestris plants that had parasitized a host plant. Shoot segments were then attached to new inflorescence stems of 4–5-week-old A. thaliana using surgical tape (Micropore™ Surgical Tape, 3M Company) and parasitism induced under blue light at 25°C as for seedlings. The process of parasitism was recorded by time-lapse imaging (TLC200, Brinno). The time at which coiling of C. campestris around the host plant was complete was designated as 0 hours after coiling (hac).
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