Bacto yeast

The materials and reagents in this study were obtained from the following sources: Gelatin from porcine skin (gel strength 300 Type A) was purchased from Sigma Aldrich Ltd (St. Louis, MO, USA); Doripenem and Flumarin were purchased from Shionogi & Co., Ltd (Osaka, Japan); Omegacin, Streptomycin sulfate, and Penicillin G potassium were purchased from Meiji Seika Pharma Cooperation Ltd (Tokyo, Japan); human umbilical vein endothelial cells (HUVECs) were obtained from Kurabo Industries Ltd (Osaka, Japan); collagenase, 2-(N-morpholino)ethanesulfonic acid (MES), D (+) glucose, agar, N-hydroxysuccinimide, HEPES, and 10% formalin were purchased from Wako Pure Chemical Cooperation (Osaka, Japan); Bacto tryptone, a pancreatic digest of casein, was purchased from Beston, Dickinson, and Company (Durham, NC, USA); Bacto yeast was purchased from Sigma Aldrich Co., Ltd (St. Louis, MO, USA); Fasting Blood Sugar (FBS) was purchased from Capricorn Scientific GmbH (Ebsdorfergrund, Germany); EGM-2 was from Lonza Walkersville MD (Walkersvile, MD, USA), and the WST-8 assay kit was obtained from Dojindo (Kumamoto, Japan). All other chemicals were of an analytical grade. Nine-week-old SD rats were obtained from SLC., Co., Ltd (Fukuoka, Japan).