The levels of IL-6 and HMGB1 in AF and cell culture medium (conditioned medium) were determined by an ELISA assay kit (Cusabio, Wuhan, China) according to the manufacturer’s instructions. The levels of IL-1ß and IL-8 in culture medium were determined by an ELISA assay kit (Cusabio and RayBiotech, Norcross, GA, USA, respectively) according to the manufacturer’s instructions.
Elisa assay kit
Manufactured by Cusabio
Sourced in China, United States
The ELISA assay kit is a laboratory equipment designed to perform enzyme-linked immunosorbent assay (ELISA) testing. ELISA is a widely used analytical technique for the detection and quantification of various analytes, such as proteins, hormones, or antibodies, in a sample. The kit provides the necessary components, including reagents and microplates, to conduct ELISA experiments in a standardized and efficient manner.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Bio-Rad (2 mentions)
Elisa assay kits, by Neobioscience (1 mentions)
Mitosox red staining, by Thermo Fisher Scientific (1 mentions)
Ros assay kit, by Beyotime (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Colorimetric assay kit, by Abcam (1 mentions)
Colorimetric assay kit, by Spinreact (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Il 1α, by Cusabio (1 mentions)
Centrifuge 5415d, by Eppendorf (1 mentions)
23 protocols using elisa assay kit
1
Cytokine Levels Determination by ELISA
2
Serum Biomarkers and Liver Oxidative Stress
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The levels of alanine transaminase (ALT) and aspartate transaminase (AST) in the serum were quantitated using commercial assay kits (Jiancheng, Nanjing, China) according to the manufacturer’s instructions. Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) activity in the liver tissues were determined using a corresponding commercial assay kit (Jiancheng, Nanjing, China). TNF-α, IL-6, and MCP-1 concentrations in the plasma were measured by the ELISA assay kits (Neobioscience, Shenzhen, China), and the serum MCP-3 level was detected with an ELISA assay kit (CUSABIO, Wuhan, China).
3
Hepatic Lipid Profiling Assay
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Levels of TC and TG in the liver were measured using a TC and TG Kit (Rongsheng Biological Co., Ltd., China). The levels of AST and ALT in serum were assessed using ELISA Assay Kit (CUSABIO, Nanjing, China) in accordance with the manufacturer’s instructions.
4
Glucose Metabolism Assessment in Mice
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The blood glucose of each mouse was measured weekly via the tail vein. At the end of the experiment, the oral glucose tolerance of each mouse was assessed via the tail vein. All blood glucose levels via the tail vein were determined using a blood glucose test strip (ACON Biotech (Hangzhou) Co. Ltd, Hangzhou, China). Overnight urine samples were collected from each mouse in the metabolic cage, and urinary glucose levels were measured using a glucose determination kit (Shanghai Rongsheng Biotech Co. Ltd, Shanghai, China) in the sixth week.
After anesthetized, blood samples from the femoral artery were collected and placed at 4 °C for 2 h, and then centrifuged at 3, 000 rpm for 10 min to obtain the serum. The GSP level was determined using a glycosylated serum protein assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The insulin level was determined using an ELISA assay kit (CUSABIO BIOTECH CO. LTD, Wuhan, China).
After anesthetized, blood samples from the femoral artery were collected and placed at 4 °C for 2 h, and then centrifuged at 3, 000 rpm for 10 min to obtain the serum. The GSP level was determined using a glycosylated serum protein assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The insulin level was determined using an ELISA assay kit (CUSABIO BIOTECH CO. LTD, Wuhan, China).
5
Quantifying Inflammatory Cytokines in NRK-52E Cells
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The culture medium supernatant of NRK-52E cells was collected and centrifuged at 1000 × g for 10 min at 4 °C. Subsequent to the removal of cell debris and other impurities, the supernatant was aliquoted into small EP tubes and stored at − 20 °C to avoid repeated freezing and thawing. The levels of IL-1β, IL-6 and TNF-α in the cell supernatant were then measured under the instructions of the corresponding ELISA assay kit (Cusabio Biotech Co., Ltd, China).
6
Measuring Cytokine Levels in Rat Serum
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Whole blood samples of rats were obtained through eyeball extraction and stored at room temperature for 2 h, followed by centrifugation at 1500 × g for 10 min to collect the serum. The levels of IL-1β, TNF-α, or IL-6 were detected by using the enzyme-linked immunoassay (ELISA) assay kit (CUSABIO, Wuhan) with serum or hippocampus tissue lysate. Briefly, 50 μL of the samples or standards were added to each well, followed by the addition of a 50-μL of antibody cocktail that was incubated at 37 °C for 1 h. At the end of the incubation, the mixture was discarded, and the plate was washed with PBS. The TMB development solution was added for 10 min. Finally, 100 µL of the stop solution was added, and the optical density (OD) at 450 nm was recorded with a luminometer.
7
Apoptosis Quantification in Bladder Cancer Cells
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Bladder cancer 5637 and T24 cells were transfected with Cas13a vectors in petri plates. Twenty‐hour hours after transfection, apoptosis was detected by measuring the activity of caspase 3 using the caspase 3 enzyme‐linked immunosorbent assay (ELISA) assay kit (Cusabio, Wuhan, China) according to the manufacturer's instructions. OD values were measured at 450 nm using a microplate reader (Bio‐Rad).
8
Quantifying MLCK Protein Levels
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The protein levels of MLCK in HPAF-II cells were detected by a MLCK enzyme-linked immune sorbent assay (ELISA) assay kit (CUSABIO, Wuhan, China) following the manufacturer's instructions. Protein standards were always utilized for MLCK quantification.
9
Caspase 3 Activity Quantification
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The level of the cell apoptosis was detected by measuring the activity of caspase 3 by using the caspase 3 enzyme-linked immunosorbent assay (ELISA) assay kit (Cusabio, Wuhan, China), and the specific operation steps mainly according to the instructions of manufacturer. We measured the OD values by using a microplate reader (Bio-Rad).
10
Quantifying Oxidative Stress in Cardiomyocytes
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MitoSOX Red staining (M36008, Invitrogen, USA) was used to assess mitochondrial ROS levels in cardiac tissues. Mitochondrial ROS was detected in H9C2 cells using a ROS assay kit (S0033, Beyotime, China), and then detected using flow cytometry according to the protocol provided by the manufacturer. ATP concentration in the cardiomyocytes was measured using an ATP assay kit (S0026, Beyotime, China), following the manufacturer's instructions. The results of each assay were normalized by protein concentration as described previously [28 (link)]. The results were shown as mmol/mg protein. The levels and activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), Catalase (CAT) and malondialdehyde (MDA) were measured according to the ELISA kit instructions to further assess the level of oxidative stress (2 R-KMLJr30259, 2M-KMLJM220779 m, 2M-KMLJM220658 m, 2M-KMLJM219464 m, Camilo, China). The levels of 4-hydroxy-2-nonenal (4-HNE), 3-nitrotyrosine (3-NT), and protein carbonyl in myocardial tissues were determined using an ELISA assay kit (
CSB-E13411-13 m, Cusabio, China), and were expressed in nmol/mg protein as measured according to the manufacturer's protocol.
CSB-E13411-13 m, Cusabio, China), and were expressed in nmol/mg protein as measured according to the manufacturer's protocol.
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