Lymphocyte separation medium, aprotinin, dimethyl sulfoxide (DMSO) and solvents for HPLC and LC/MS were purchased from Thermo Fisher Scientific (Ottawa, Ontario, Canada). Dextran, adenosine deaminase, leupeptin and potassium phosphate were obtained from Sigma-Aldrich Canada (Oakville, Ontario, Canada). HBSS was purchased from Wisent Bioproducts (St-Bruno, Quebec, Canada). 19-OH-prostaglandin (PG) B2, PGB2, PGB2-D4 and 17-octadecynoic acid (17-ODYA) were purchased from Cayman Chemicals (Ann Arbor, Michigan, USA). PF-4708671 was obtained from Abcam (Cambridge, Massachusetts, USA) and LY2584702 from Selleckchem (Houston, Texas, USA). Thapsigargin was obtained from Tocris Bioscience (Ellisville. Missouri, USA). LTB4 was a generous gift from Dr Louis Flamand (Université Laval, Québec City, Canada). Recombinant CYP4F3A and the NADPH regenerating system were purchased from Corning (Corning, New York, USA). Protease and phosphatase inhibitor cocktail tablets were purchased from Roche (Laval, Quebec, Canada). Primary (anti-phospho-S6 #2211 and anti-S6 #2317) and secondary antibodies were obtained from Cell Signaling (Danvers, Massachusetts, USA). The enhanced chemiluminescent (ECL) substrate was obtained from Millipore Canada Ltd (Toronto, Ontario, Canada).
Thapsigargin
Manufactured by Bio-Techne
Sourced in United Kingdom, United States
Thapsigargin is a selective inhibitor of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump. It disrupts calcium homeostasis within the cell by blocking the uptake of calcium into the endoplasmic reticulum.
Automatically generated - may contain errors
Lab products found in correlation
U73122, by Bio-Techne (11 mentions)
Ryanodine, by Bio-Techne (10 mentions)
Fura 2 am, by Thermo Fisher Scientific (9 mentions)
Nifedipine, by Merck Group (6 mentions)
Ionomycin, by Bio-Techne (6 mentions)
Nifedipine, by Bio-Techne (5 mentions)
Tunicamycin, by Bio-Techne (5 mentions)
Gsk 7975a, by Aobious (4 mentions)
Bapta am, by Bio-Techne (4 mentions)
Gf109203x, by Bio-Techne (4 mentions)
Adenosine triphosphate (atp), by Merck Group (4 mentions)
Nicardipine, by Merck Group (4 mentions)
Fluo 4 am, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Forskolin, by Bio-Techne (3 mentions)
Bicuculline, by Bio-Techne (3 mentions)
Isradipine, by Bio-Techne (3 mentions)
Nnc 55 0396, by Alomone (3 mentions)
Xestospongin c, by Bio-Techne (3 mentions)
Caffeine, by Merck Group (3 mentions)
91 protocols using thapsigargin
1
Lymphocyte Isolation and Lipid Mediator Analysis
2
Dissecting Apoptosis Signaling Pathways
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G1 was purchased from Tocris (Wiesbaden-Nordenstadt, Germany), dissolved (5 mM) in dimethyl sulfoxide (DMSO) (Roth, Karsruhe, Germany) and stored at −20 °C; Thapsigargin, SP600125, GSK2606414 were also purchased from Tocris. Indo-1 AM was from Thermo Fisher Scientific (Waltham, MA, USA). zVAD-fmk was bought from Santa Cruz (Santa Cruz, CA, USA). SB203580 and Kira6 were purchased from MERCK Millipore (Darmstadt, Germany). All substances were dissolved in DMSO. Antibodies were obtained from the following commercial sources: caspase 9 (Ca# 9502), cleaved PARP (Ca# 9541), IRE1α (Cat# 3294), PERK (Cat# 3192), eIF2α (Cat# 5234), phospho-eIF2α (Cat# 3398), BiP GRP78 (Ca# 3177), CHOP (Cat# 2895), p38 MAPK (Ca# 9212), phospho-p38 MAPK (Ca# 4511), phospho-SAPK/JNK (Ca# 4668), caspase 3 (Ca# 9662), BCL-2 (Ca# 2872), Cell Signaling (Danvers, MA, USA); ATF6 (Cat# 73500), BioAcademia (Osaka, Japan); puromycin (Ca# MABE343), cylophilin D (Ca# AP1035), MERCK Millipore (Darmstadt, Germany); phospho-IRE1α (Cat# NBP2-50067), Novus Biologicals (Littleton, CO, USA); cytochrome c (Ca# 556433), BD Biosciences (Franklin Lakes, NJ, USA); β-actin (Cat# A5441, Sigma-Aldrich (Steinheim, Germany)). Secondary, peroxidase-conjugated antibodies were purchased from Dianova (Hamburg, Germany). All other chemicals of analytical grade were obtained from Sigma-Aldrich or Roth.
3
Pharmacological Inhibitors of Cellular Signaling
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Tetrodotoxin (TTX), 2-(4-chloro-2-methylphenoxy)-N-[(2-methoxyphenyl) methylideneamino]-acetamide (Ani-9), Benzbromarone, Nω-nitro-L-arginine (L-NNA), 1H-[1,2,4] Oxadiazolo [4,3-a] quinoxalin-1-one (ODQ), and Thapsigargin were purchased from Tocris Bioscience (Ellisville, Missouri, USA). GSK 7975 A was purchased from Aobious (Gloucester, MA).
4
Pharmacology of Neurotransmitter Signaling
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Salts and general reagents were purchased from Sigma (St. Louis, MO, USA); GYKI 53655, D-AP5, NBQX, bicuculline, Rp-Br-cAMP, H-89, forskolin, philanthotoxin, ryanodine, thapsigargin, kainate, Pertussis toxin, CMZ and W-7 were obtained from Tocris (Bristol, UK).
5
Perfusion of Tissues with Oxygenated KRB Solution
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All tissues were perfused continuously with KRB solution containing (mmol/L): NaCl, 5.9; NaHCO3, 120.35; KCl, 1.2; MgCl2, 15.5; NaH2PO4,1.2; CaCl2, 2.5; and glucose, 11.5. The KRB solution was warmed to a physiological temperature of 37 ± 0.3°C and bubbled with a mixture of 97% O2 – 3% CO2. For experiments utilizing external solutions with 0 [Ca2+]o, CaCl2 was omitted and 0.5 mM ethylene glycol-bis (β-aminoethyl ether)-N, N, N’, N’–tetraacetic acid (EGTA) was added to the solution. NNC 55–0396 and TTA-A2 were purchased from Alomone Labs (Jerusalem, Israel). 2-aminoethyl-diphenylborinate (2-APB), tetracaine, nicardipine, pinacidil was purchased from Millipore-Sigma (St. Louis, Missouri, USA). Thapsigargin, isradipine, Z-944, CPA and ryanodine were purchased from Tocris Bioscience (Ellisville, Missouri, USA). GSK 7975A was purchased from Aobious (Aobious INC, MA, USA), and xestospongin C (XeC) was purchased from Cayman Chemical (Michigan, USA).
6
Electrophysiological Recordings with Diverse Solutions
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Normal aCSF was used in most cases for in vitro electrophysiological recordings. Ca2+-free solution was made by removing Ca2+ chloride from the recording solution and replacing it with an equimolar concentration of magnesium chloride. Low-Na+ solution was made by substituting equimolar concentrations of Na+ chloride by choline chloride. All solutions were oxygenated with 95% O2/5% CO2. All salt compounds, TEA (#T2265); TPPO (#T84603), L.A. (#L1376), BAPTA (#A9801); Caffeine (#C0750), NMA (#M2137), and 5-HT (#S2805) were obtained from Sigma-Aldrich. TTX (#1078), Chelerythrin (#1330), U73122 (#1268), Xestospongin-C (#1280), 9-Phenanthrol (#4999), Dantrolene (#0507), and Thapsigargin (#1138) were obtained from Tocris Bioscience. Dantrolene, Thapsigargin, Xestospongin-C, U73122, and 9-Phenantrol were dissolved in dimethylsulfoxide (DMSO) and added to the aCSF (final concentration of DMSO: 0.05–0.1%). L.A. was dissolved in ethanol and added to aCSF (final concentration of ethanol: 0.05–0.1%). Control experiments showed no effects of the vehicle. The other drugs were dissolved in water and added to the aCSF.
7
Pharmacological Modulation of Neural Signaling
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ACET, picrotoxin, TPMPA, strychnine, DNQX, W-7, calmidazolium (CMZ), CALP1, ML-9, KN-62, MMPX, ascomycin, BAPTA-AM, thapsigargin (Tg), YM-58483 were obtained from Tocris. L-AP4 was purchased from Tocris or Cayman. Meclofenamic acid (MFA) and I4AA were obtained from Sigma. TTX was purchased from Alomone Labs. Drugs were dissolved in dimethylsulfoxide (DMSO) where appropriate and then diluted into the bath solution. The final concentrations of DMSO were <0.1% (v/v) in all experiments.
8
Pharmacological Modulation of Ion Channels
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4-aminopyridine (4-AP) and pyruvate were purchased from Sigma (St. Louis, MO, USA). Hexahydro-1-[(5-iodo-1-naphthalenyl) sulfonyl]-1H-1,4-diazepine hydrochloride (ML-7) and (S)-(+)-2-Methyl-1-[(4-methyl-5-isoquinolinyl)sulfonyl]-hexahydro-1H-1,4-diazepine dihydrochloride (H-1152) were purchased from Calbiochem (San Diego, CA, USA). Ethyleneglycol-bis (β-aminoethyl)-N,N,N',N'-tetraacetic acid (EGTA) was purchased from Dojin (Kumamoto, Japan). Tetrodotoxin (TTX) was purchased from Wako (Osaka, Japan). D-(−)-2-amino-5-phosphonopentanoic acid (APV), bicuculline, (S)-(−)-blebbistatin, (R)-(+)-blebbistatin, thapsigargin and nifedipine were purchased from Tocris (Ellisville, MO, USA).
9
Treatment of Cellular Stress Pathways
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Arsenite (sodium Arsenite, Millipore Sigma, catalog no. S7400) was dissolved in water at 100 mmol/L, and the treatment was performed at a concentration of 300 μmol/L for 3 hours (50 (link)). MG132 (Enzo, catalog no. BML-PI102–0025) was dissolved in DMSO at 20 mmol/L, and the treatment was performed at a concentration of 10 μmol/L for 6 hours. ISRIB (trans-ISRIB, Tocris, catalog no. 5284) was dissolved in DMSO at 5 mmol/L and treated at a concentration of 1 μmol/L. Thapsigargin (Tocris, catalog no. 1138) was dissolved in DMSO at 5 mmol/L and treated at a concentration of 1 μmol/L for 12 hours unless otherwise indicated. Staurosporine (Tocris, catalog no. 1285), everolimus (Tocris, catalog no. 6188), and chloroquine (chloroquine diphosphate, Tocris, catalog no. 4109) were all dissolved in DMSO, and the treatment was performed at a concentration of 1 μmol/L, 5 μmol/L, and 100 μmol/L, respectively, for 12 hours.
10
Pharmacological Reagents Procurement
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Ouabain was purchased from the International Laboratory USA (South San Francisco, CA, USA). 2-2-Aminoethoxydipheylborate (2-APB) and thapsigargin were purchased from Tocris (Bristol, UK). Nifedipine, ryanodine, zatebradine, and all chemical reagents used to setup buffer solutions were purchased from Sigma.
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