The BLL was quantified by using an inductively coupled plasma mass spectrometry with triple quadrupole technology (iCAP™ TQ ICP-MS, Thermo Fisher Scientific, Bremen, Germany). The EDTA blood was homogenized for 10 min by mechanical shaking; thereafter blood plasma (1.0 ml) was gravimetrically diluted (in precleaned polypropylene bottles kept in 2% nitric acid for 72 h) with 0.5% m/m nitric acid (Fisher Scientific) and 2% m/m tetramethylammonium hydroxide (Merck, Sigma Aldrich) in ultrapure water. A calibration blank, a series of standards, and a quality control were prepared by using the same procedure. All samples and standards were spiked with an internal standard mix (10 μg/L of 209Bi). The sample digests were filtered with Wattman paper several times to obtain a clear solution. The diluted digests were measured directly by ICP-MS, and the concentration of Pb in blood was quantified as μg/dl.
Nitric acid
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Belgium, Germany, France, India, Spain, Malaysia, Canada, Australia
Nitric acid is a colorless to slightly yellow, fuming, and highly corrosive liquid chemical. It is commonly used in laboratories for various analytical and synthetic applications.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Thermo Fisher Scientific (53 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (25 mentions)
Sulfuric acid, by Thermo Fisher Scientific (17 mentions)
Methanol, by Thermo Fisher Scientific (17 mentions)
Silver nitrate, by Merck Group (12 mentions)
Sodium borohydride, by Merck Group (10 mentions)
Hydrogen peroxide, by Thermo Fisher Scientific (10 mentions)
Formic acid, by Thermo Fisher Scientific (9 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (8 mentions)
Acetone, by Thermo Fisher Scientific (7 mentions)
Potassium hydroxide, by Thermo Fisher Scientific (7 mentions)
Acetonitrile, by Thermo Fisher Scientific (7 mentions)
Agilent 7500i, by Agilent Technologies (6 mentions)
Ethanol, by Thermo Fisher Scientific (6 mentions)
Hydrofluoric acid, by Thermo Fisher Scientific (6 mentions)
Sodium hydroxide (naoh), by Merck Group (6 mentions)
Chloroform, by Thermo Fisher Scientific (5 mentions)
Sodium carbonate, by Thermo Fisher Scientific (5 mentions)
Nitric acid, by Merck Group (5 mentions)
Sodium bicarbonate, by Thermo Fisher Scientific (4 mentions)
193 protocols using nitric acid
1
Quantification of Lead in Blood
2
Measuring Cellular Copper Concentration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
YST cells were transfected with 2 µg of the ATP7B plasmids as described above. After 16 h of transfection, the cells were treated with 100 μM CuCl2 for 24 h and then collected by centrifugation at 10,000 rpm for 10 min. Approximately 106 cells were collected from each well and then washed once with ultrapure water. The cell pellets were resuspended in 100 µL of concentrated nitric acid (trace element grade, Fisher Scientific) and heated at 90 °C for 2 h. The samples were cooled to RT and then diluted in 1.4 mL of 1% nitric acid. The volume of the sample was determined before ICP-MS measurements were taken. The concentration of Cu was normalized to cell count.
3
Elemental Analysis of Wine Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For determination of the elemental content in wine (see Table 2 ), samples were diluted 10 times with water containing 2% (v/v) nitric acid (Merck, Germany). Standards were prepared with 1% (v/v) ethanol and 2% (v/v) nitric acid in order to provide the same concentrations of ethanol and nitric acid as the samples [14 (link),15 (link),16 (link),17 (link),18 ,19 (link)].
The elements were determined using inductively coupled plasma with optical emission spectrometry (ICP-OES, iCAP 6500 Duo Thermo Scientific, Llanthony Rd, Gloucester GL2 8DN, Highnam, UK) and inductively coupled plasma quadrupole mass spectrometry (ICP-QMS, Thermo Scientific, Xseries 2, Hemel Hempstead, UK). ICP-OES was applied for K, Na, Mg, Ca, Rb, and Fe examination, while the remaining 14 elements were analyzed using ICP-QMS. For elemental determination, a multi-element plasma standard solution 4, Specpure, containing 1 g/L of 22 elements were used for calibration [19 (link)].
The elements were determined using inductively coupled plasma with optical emission spectrometry (ICP-OES, iCAP 6500 Duo Thermo Scientific, Llanthony Rd, Gloucester GL2 8DN, Highnam, UK) and inductively coupled plasma quadrupole mass spectrometry (ICP-QMS, Thermo Scientific, Xseries 2, Hemel Hempstead, UK). ICP-OES was applied for K, Na, Mg, Ca, Rb, and Fe examination, while the remaining 14 elements were analyzed using ICP-QMS. For elemental determination, a multi-element plasma standard solution 4, Specpure, containing 1 g/L of 22 elements were used for calibration [19 (link)].
4
Trace Metal Enrichment Membrane Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Polyethersulfone (PES) microfiltration membranes (0.45 μm pore diameter, 47 mm diameter) were purchased from MilliporeSigma (Burlington, USA). Glycidyl methacrylate (≥97.0%), lanthanum(III) nitrate hexahydrate (99.999% trace metal basis), cerium(III) chloride heptahydrate (99.9% trace metal basis), neodymium(III) nitrate hexahydrate (99.9% trace metal basis), zinc perchlorate hexahydrate, calcium chloride (anhydrous, Redi-Dri, ≥97%), sodium standard for ICP (1,000 mg/L ± 2 mg/L), calcium standard for ICP (1,000 mg/L ± 2 mg/L), and magnesium standard for ICP (1,000 mg/L ± 2 mg/L) were purchased from Sigma-Aldrich. Magnesium chloride hexahydrate (99–102%, ACS grade) was purchased from VWR Life Science. L-lysine (98%, powder) was purchased from Alfa Aesar. Ethyl alcohol (absolute, 200 proof, ≥99.5%, ACS Reagent grade) was purchased from Acros Organics. Denatured Ethyl alcohol (88–91%), sodium chloride (≥99%, certified ACS grade, crystalline), nitric acid (68–70%, certified ACS plus grade), and hydrochloric acid (36.5–38%, certified ACS plus grade) were purchased from Fisher Chemical. Arsenazo (III) was purchased from Pointe Scientific. Sodium hydroxide (10 N, biotech reagent grade) was purchased from Avantor. Deionized water (DI water) was made from a RiOS-DI 3 water purification system (MilliporeSigma, Burlington, MA, USA).
5
Antibiotic Sensitivity of Multidrug-Resistant Salmonella
6
Synthesis and Characterization of Iron Nanoparticles
7
Assessing Metal Levels in Bone Broths
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three animal bone broth-based street foods were obtained from local stores in Douliu city (central Taiwan) to evaluate the levels of essential and toxic metals in broths, and the health risks that would be associated with their ingestion. These foods included pork rib chops that had been stewed in Chinese medicinal herb broth (PR, n = 7), beef noodle (Chinese-style noodle with stewed beef in bovine bone broth, BN, n = 6) and tonkotsu ramen (Japanese-style noodle in pork bone broth, TR, n = 6), all of which are readily available. For simplicity, only the liquid portion (broth) of the foods was assessed. After the samples were cool, the soups were filtered using a 20-mesh gauze; the volume and pH level were recorded, and any grease was removed. The remaining contents were acidified by adding 3 ml of diluted (1:1) nitric acid (~67%, Fisher) per litre of broth, and kept at 4°C until analysis.
8
Quantifying Stent Encrustation Composition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experimental containers were prewashed with
1% nitric acid (Fisher Scientific, Hampton, NH) before ultra-trace
elemental analysis via inductively coupled plasma mass spectrometry
(ICP-MS). 11 week dried, encrusted stent specimens were submerged
in 1 mL of 1% HNO3 (v/v), followed by ultrasonication for
1 h using a Branson ultrasonic cleaner (Branson Ultrasonics Corp.,
Danbury, CT) to agitate, extract, and dissolve the encrusted minerals
on the stents. Each sonicated sample was further diluted 100-fold
in 1% HNO3 to achieve a total dissolved solid of <1
weight percent (wt %) and a final volume of 10 mL before ICP-MS analysis.
The presence of magnesium (Mg), calcium (Ca), and phosphorus (P) was
measured by using an iCAP RQ inductively coupled plasma mass spectrometer
with a Teledyne laser ablation (LA) system (Thermo Scientific, Waltham,
MA, USA). Standard solutions of Mg, Ca, and P (Inorganic Ventures,
Christiansburg, VA, USA) were used to generate a standard curve (100,
50, 20, and 0 ppm). One-way ANOVA was performed using GraphPad Prism
(Dotmatics, Boston, MA, USA) to compare the amount of encrusted deposit
on each stent material.
1% nitric acid (Fisher Scientific, Hampton, NH) before ultra-trace
elemental analysis via inductively coupled plasma mass spectrometry
(ICP-MS). 11 week dried, encrusted stent specimens were submerged
in 1 mL of 1% HNO3 (v/v), followed by ultrasonication for
1 h using a Branson ultrasonic cleaner (Branson Ultrasonics Corp.,
Danbury, CT) to agitate, extract, and dissolve the encrusted minerals
on the stents. Each sonicated sample was further diluted 100-fold
in 1% HNO3 to achieve a total dissolved solid of <1
weight percent (wt %) and a final volume of 10 mL before ICP-MS analysis.
The presence of magnesium (Mg), calcium (Ca), and phosphorus (P) was
measured by using an iCAP RQ inductively coupled plasma mass spectrometer
with a Teledyne laser ablation (LA) system (Thermo Scientific, Waltham,
MA, USA). Standard solutions of Mg, Ca, and P (Inorganic Ventures,
Christiansburg, VA, USA) were used to generate a standard curve (100,
50, 20, and 0 ppm). One-way ANOVA was performed using GraphPad Prism
(Dotmatics, Boston, MA, USA) to compare the amount of encrusted deposit
on each stent material.
9
Synthesis and Characterization of Potassium Ferrate
10
Copper Nanoparticle Synthesis Protocols
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!